Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh.
School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.
Curr Microbiol. 2024 Nov 29;82(1):22. doi: 10.1007/s00284-024-03996-z.
Multidrug-resistant bacteria, particularly extended-spectrum-beta-lactamase-producing (ESBL) bacteria, pose a significant global public health challenge. Klebsiella pneumoniae (KPN) is frequently implicated in cases of this resistance. This study aimed to investigate the presence of drug and metal resistance genes in clinical K. pneumoniae isolate Kp04 and comparative genomics of clinical KPN isolates characterized from Bangladesh. A total of 12 isolates were collected. Disk-diffusion assay showed that all five isolates were resistant to 14 out of 21 tested antibiotics and sensitive to only three-tigecycline, imipenem, and meropenem. KPN Kp04 was positive for both bla and bla ESBL genes in PCR. All five isolates produced PCR amplicons of the correct size for ampicillin (ampC), tetracycline (tetC), fluoroquinolone (qnrS), and aminoglycoside (aadA) resistance genes. The whole genome of Kp04 was sequenced using the MiSeq Platform (V3 kit, 2 × 300 cycles). We utilized different databases to detect Antibiotic-Resistant Genes (ARGs), virulence factor genes (VFGs), and genomic functional features of the Kp04 strain. Whole-genome sequencing identified 75 ESBL, virulence, and multiple drug-resistant (MDR) genes including bla, tetA, oqxA, oqxB, aadA, sul1-5, and mphA in KPN Kp04 isolate. Pan-genomic analysis of 43 Bangladeshi KPN isolates showed similarities between Dhaka and Chattogram isolates regarding virulence and antibiotic-resistant genes. Our results indicate the transmission of similar virulent KPN strains in Dhaka and Chattogram. This study would provide valuable information about drug sensitivity, antibiotic, and metal resistance features of K. pneumoniae circulated among hospitalized patients in Bangladeshi megacities.
多药耐药细菌，特别是产超广谱β-内酰胺酶（ESBL）的细菌，对全球公共卫生构成重大挑战。肺炎克雷伯菌（Klebsiella pneumoniae，KPN）经常与这种耐药性有关。本研究旨在调查临床分离的肺炎克雷伯菌 Kp04 中药物和金属耐药基因的存在，并对来自孟加拉国的临床 KPN 分离株进行比较基因组学分析。共收集了 12 株分离株。药敏纸片扩散法显示，5 株分离株均对 21 种受试抗生素中的 14 种耐药，仅对 3 种抗生素敏感，分别为替加环素、亚胺培南和美罗培南。PCR 显示 KPN Kp04 同时携带 bla 和 bla ESBL 基因。5 株分离株均产生大小正确的氨苄西林（ampC）、四环素（tetC）、氟喹诺酮（qnrS）和氨基糖苷（aadA）耐药基因 PCR 扩增子。使用 MiSeq 平台（V3 试剂盒，2×300 个循环）对 Kp04 全基因组进行测序。我们利用不同的数据库来检测 Kp04 菌株的抗生素耐药基因（ARGs）、毒力因子基因（VFGs）和基因组功能特征。全基因组测序鉴定出 75 个 ESBL、毒力和多药耐药（MDR）基因，包括 bla、tetA、oqxA、oqxB、aadA、sul1-5 和 mphA，这些基因均存在于 KPN Kp04 分离株中。对 43 株孟加拉国 KPN 分离株的全基因组分析显示，达卡和吉大港分离株的毒力和抗生素耐药基因相似。我们的结果表明，在达卡和吉大港传播着类似的毒力 KPN 菌株。本研究将为孟加拉国大城市住院患者中流行的肺炎克雷伯菌的药敏、抗生素和金属耐药特征提供有价值的信息。
