Tanni Afroza Akter, Sultana Nahid, Ahmed Wazir, Hasan Md Mahbub, Hossain Md Shakhawat, Noyon Sajjad Hossain, Hossain Md Mobarok, Mannan Adnan
Department of Genetic Engineering & Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chattogram-4331, Bangladesh.
Disease Biology and Molecular Epidemiology Research Lab, Biotechnology Research & Innovation Centre (BRIC), Department of Genetic Engineering & Biotechnology, University of Chittagong, Chattogram, Bangladesh.
Can J Infect Dis Med Microbiol. 2022 Sep 30;2022:7071009. doi: 10.1155/2022/7071009. eCollection 2022.
Multidrug-resistant (MDR) clones of () have been increasingly documented in community-acquired and nosocomial infections all around the globe. Extended-spectrum -lactamases (ESBLs) are a rapidly evolving group of -lactamase enzymes derived from SHV genes by mutations. This research work aimed to investigate and analyze the widespread prevalence of antibiotic resistance in different areas of the southern part of Bangladesh.
This particular study was executed and implemented by using 501 clinical samples or isolates from two different hospitals in Chattogram. The disk diffusion method was used to detect 's sensitivity to 16 antibiotics in a drug susceptibility test. By using the PCR technique, the widespread prevalence of antibiotic-resistant gene bla was studied. Sequencing along with phylogenetic analysis was utilized to verify isolates with the bla gene.
Almost all of the isolates were spotted to be antibiotic-resistant. These isolates were resistant to -lactams, aminoglycosides, and quinolones at high levels. The spatial analysis displayed that infections involving were more common in the urban areas (70%) than in the rural areas (30%). Neonates had substantially higher levels ( < 0.001) of resistance to multidrug than other age groups. Cefepime was identified as the most frequent antibiotic-resistant to all age groups (56.68%). The highest numbers of resistant isolates (36.92%) were found in urine samples. The ESBL gene bla was found in 38% isolates.
The significant frequency of MDR harboring -lactamases and AMR genes strongly suggests the requirement to develop effective antimicrobial resistance control and prevention measures in Bangladesh.
全球范围内，社区获得性感染和医院感染中越来越多地发现了（）的多重耐药（MDR）克隆株。超广谱β-内酰胺酶（ESBLs）是一组通过突变从SHV基因衍生而来的快速进化的β-内酰胺酶。本研究旨在调查和分析孟加拉国南部不同地区抗生素耐药性的广泛流行情况。
本研究使用了来自吉大港市两家不同医院的501份临床样本或分离株。在药敏试验中，采用纸片扩散法检测对16种抗生素的敏感性。通过PCR技术研究抗生素耐药基因bla的广泛流行情况。利用测序和系统发育分析来验证携带bla基因的分离株。
几乎所有的分离株都被发现具有抗生素耐药性。这些分离株对β-内酰胺类、氨基糖苷类和喹诺酮类抗生素具有高水平耐药性。空间分析显示，涉及的感染在城市地区（70%）比农村地区（30%）更常见。新生儿对多种药物的耐药水平显著高于其他年龄组（P<0.001）。头孢吡肟被确定为所有年龄组中最常见的耐药抗生素（56.68%）。尿液样本中发现的耐药分离株数量最多（36.92%）。在38%的分离株中发现了ESBL基因bla。
携带β-内酰胺酶和AMR基因的MDR的高频率强烈表明，孟加拉国需要制定有效的抗菌药物耐药性控制和预防措施。
