Investigating Antimicrobial Resistance and ESBL Producing Gene in Isolates among Neonates and Adolescents in Southern Bangladesh.

BACKGROUND

Multidrug-resistant (MDR) clones of () have been increasingly documented in community-acquired and nosocomial infections all around the globe. Extended-spectrum -lactamases (ESBLs) are a rapidly evolving group of -lactamase enzymes derived from SHV genes by mutations. This research work aimed to investigate and analyze the widespread prevalence of antibiotic resistance in different areas of the southern part of Bangladesh.

METHODS

This particular study was executed and implemented by using 501 clinical samples or isolates from two different hospitals in Chattogram. The disk diffusion method was used to detect 's sensitivity to 16 antibiotics in a drug susceptibility test. By using the PCR technique, the widespread prevalence of antibiotic-resistant gene bla was studied. Sequencing along with phylogenetic analysis was utilized to verify isolates with the bla gene.

RESULTS

Almost all of the isolates were spotted to be antibiotic-resistant. These isolates were resistant to -lactams, aminoglycosides, and quinolones at high levels. The spatial analysis displayed that infections involving were more common in the urban areas (70%) than in the rural areas (30%). Neonates had substantially higher levels ( < 0.001) of resistance to multidrug than other age groups. Cefepime was identified as the most frequent antibiotic-resistant to all age groups (56.68%). The highest numbers of resistant isolates (36.92%) were found in urine samples. The ESBL gene bla was found in 38% isolates.

CONCLUSION

The significant frequency of MDR harboring -lactamases and AMR genes strongly suggests the requirement to develop effective antimicrobial resistance control and prevention measures in Bangladesh.