RNA-seq 揭示了热应激对成年雌性鸡肌肉转录组的影响。

RNA-seq reveals changes in the transcriptome of the breast muscle of adult female chickens in response to heat stress.

机构信息

Tianjin Key Laboratory of Animal Molecular Breeding and Biotechnology, Tianjin Engineering Research Center of Animal Healthy Farming, Institute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin, 300381, China.

College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.

出版信息

BMC Genomics. 2024 Nov 29;25(1):1158. doi: 10.1186/s12864-024-11024-4.

DOI:10.1186/s12864-024-11024-4

PMID:39614141

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11607835/

Abstract

BACKGROUND

Heat stress has caused significant impacts on the poultry industry globally. Tianjin-monkey Chicken (TM) is a local naked neck chicken genetic resource in China, characterized by its heat stress resistance due to a low feather coverage.

RESULTS

We conducted heat stress stimulation tests on TM and a normal feathered chicken (Jingfen No. 6 Layer, JF), and the breast muscle tissues were collected for transcriptome sequencing. A total of 157 differentially expressed genes (DEGs) and 1435 DEGs were respectively obtained from the comparisons of JFN-vs-JFT and TMN-vs-TMT. GO enrichment analysis found that biological process (BP) terms including phospholipid homeostasis, regulation of aggrephagy, positive regulation of aggrephagy, and negative regulation of lipase activity may be closely related to heat stress resistance in JF chickens. While catabolism-related BP terms were mainly enriched for DEGs of TM, such as catabolic process, protein catabolic process and cellular catabolic process. KEGG pathway analysis showed that the MAPK signaling pathway was enriched both in TM and JF with high connectivity. In addition, some pathways with higher connectivity (Metabolic pathways, FoxO signaling pathway, TGF-beta signaling pathway and AMPK signaling pathway) may be closely associated with resistance to heat stress in JF. In Tianjin-monkey Chicken, we also identified several pathways potentially involved in heat stress regulation, including Ubiquitin mediated proteolysis, Autophagy-animal and Regulation of actin cytoskeleton. Protein-Protein Interaction Networks (PPI) for the 24 co-differentially expressed genes revealed four key genes (Klf9, Asb2, Tmem164 and Arrdc2) associated with heat stress both in JF and TM.

CONCLUSIONS

Our findings will enrich the research on heat stress resistance in chicken skeletal muscle, while also providing a theoretical basis for the genetic improvement of heat stress resistance in chickens.

摘要

背景

热应激已对全球家禽业造成重大影响。天津小麻鸡（TM）是中国的一种地方裸颈鸡遗传资源，由于羽毛覆盖率低，具有抗热应激能力。

结果

我们对 TM 和正常羽毛鸡（京粉 6 号蛋鸡，JF）进行了热应激刺激试验，采集胸肌组织进行转录组测序。JF-vs-JFT 和 TMN-vs-TMT 的比较分别获得了 157 个差异表达基因（DEG）和 1435 个 DEG。GO 富集分析发现，生物过程（BP）术语，包括磷脂稳态、自噬的调节、自噬的正调节和脂肪酶活性的负调节，可能与 JF 鸡的抗热应激密切相关。而 TM 的 DEG 主要富集于与分解代谢相关的 BP 术语，如分解代谢过程、蛋白质分解代谢过程和细胞分解代谢过程。KEGG 通路分析表明，MAPK 信号通路在 TM 和 JF 中均有较高的连通性。此外，一些具有较高连通性的通路（代谢途径、FoxO 信号通路、TGF-β信号通路和 AMPK 信号通路）可能与 JF 的抗热应激密切相关。在天津小麻鸡中，我们还鉴定了几个可能参与热应激调节的通路，包括泛素介导的蛋白质水解、自噬-动物和肌动蛋白细胞骨架的调节。24 个共同差异表达基因的蛋白质-蛋白质相互作用网络（PPI）揭示了 4 个与热应激相关的关键基因（Klf9、Asb2、Tmem164 和 Arrdc2），这些基因在 JF 和 TM 中都有表达。