Gao Shi-Jia, Li Xiao-Li, Gao Rui, Tan Wen-Hong, Li Wen, Liu Lu
College of Tiaditional Chinese Medicine, Ynnan University of Chinese Medicine, Kunming 650500, China.
The Second Afiliated Hospital of Kunming Medical University, China.
Fitoterapia. 2025 Jan;180:106315. doi: 10.1016/j.fitote.2024.106315. Epub 2024 Nov 29.
The study aimed to investigate the efficacy of Danggui Buxue Decoction (DBD) in treating primary dysmenorrhea (PD) and uncover its mechanisms, thereby validating its traditional use. Using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS), 38 compounds in DBD were identified. PD model rats were established via cold stimulation, estradiol benzoate, and oxytocin injections. DBD treatment improved behavioral parameters, reduced writhing response, and alleviated histopathological changes in uterus and ovary. Biochemical markers (PGE2, SOD and MDA, among others) were assessed using ELISA, and Western blot was employed to analyze protein levels in rat uteri. Results showed that DBD modulated these proteins, indicating its protective effects. The study provides scientific evidence for DBD's role in nourishing "qi" or enriching "blood" during PD treatment by affecting the MEK1/2/ERK1/2/NF-κB signaling pathway, thus reducing oxidative stress and proinflammatory factors in PD rats.
本研究旨在探讨当归补血汤（DBD）治疗原发性痛经（PD）的疗效并揭示其作用机制，从而验证其传统用途。采用超高效液相色谱-四极杆飞行时间质谱联用仪（UPLC-Q-TOF/MS）鉴定出DBD中的38种化合物。通过冷刺激、注射苯甲酸雌二醇和缩宫素建立PD模型大鼠。DBD治疗改善了行为学参数，减少了扭体反应，并减轻了子宫和卵巢的组织病理学变化。使用酶联免疫吸附测定（ELISA）评估生化标志物（如PGE2、SOD和MDA等），并采用蛋白质印迹法分析大鼠子宫中的蛋白质水平。结果表明DBD对这些蛋白质有调节作用，表明其具有保护作用。本研究通过影响MEK1/2/ERK1/2/NF-κB信号通路，为DBD在PD治疗中“补气”或“养血”的作用提供了科学依据，从而降低了PD大鼠的氧化应激和促炎因子水平。
