Romeiro Kaline, Gominho Luciana F, Voigt Danielle D, Mdala Ibrahimu, Rôças Isabela N, Siqueira José F
Postgraduate Program in Dentistry, University of Grande Rio (UNIGRANRIO), Rio de Janeiro, Rio de Janeiro, Brazil.
Department of Restorative Dentistry, Federal University of Paraíba (UFPB), João Pessoa, Paraíba, Brazil.
J Endod. 2025 Feb;51(2):157-163. doi: 10.1016/j.joen.2024.11.012. Epub 2024 Nov 29.
This study evaluated the effectiveness of chemomechanical preparation associated with intracanal medication in reducing the total bacterial load of infected root canals in cancer patients undergoing antineoplastic treatment. The prevalence, levels, and response to treatment of Streptococcus and Actinobacteria species were also assessed.
The root canals of teeth with apical periodontitis from oncological and control patients were treated using rotary instrumentation, 2.5% NaOCl irrigation, and 2-week calcium hydroxide medication (n = 23/group). Bacteriological root canal samples were taken before (S1) and after chemomechanical preparation (S2), and after intracanal medication (S3). DNA extracted from the samples was subjected to 16S rRNA gene-based quantitative real-time polymerase chain reaction assays for evaluation of the treatment effects on total bacteria, Streptococcus species, and members of the Actinobacteria phylum.
All S1 samples were positive for bacteria. S1-S2 and S1-S3 bacterial reduction values were highly significant (P < .01). Although bacterial counts were reduced from S2 to S3, the decrease was not statistically significant (P > .05). Intergroup comparisons showed no significant differences in S1, S2 and S3 counts (P > .05). In the oncological group, 77% of the cases were positive for bacteria in S2, and 68% in S3. In the control group, corresponding figures were 65% for both S2 and S3. There were no significant differences between groups as for streptococci and actinobacteria prevalence, levels, and response to treatment (P > .05).
Root canal treatment using rotary instruments, NaOCl irrigation, and calcium hydroxide medication was highly effective in reducing the bacterial populations in infected teeth with apical periodontitis from cancer patients, with no significant differences to controls.
本研究评估了化学机械预备联合根管内用药在降低接受抗肿瘤治疗的癌症患者感染根管内细菌总数方面的有效性。还评估了链球菌属和放线菌属的患病率、水平及对治疗的反应。
使用旋转器械、2.5%次氯酸钠冲洗和2周的氢氧化钙用药对肿瘤患者和对照患者患有根尖周炎的牙齿根管进行治疗（每组n = 23）。在化学机械预备前（S1）、化学机械预备后（S2）和根管内用药后（S3）采集根管细菌学样本。从样本中提取的DNA进行基于16S rRNA基因的定量实时聚合酶链反应分析，以评估治疗对总细菌、链球菌属和放线菌门成员的影响。
所有S1样本细菌检测均为阳性。S1至S2和S1至S3的细菌减少值非常显著（P <.01）。虽然从S2到S3细菌计数有所减少，但下降无统计学意义（P >.05）。组间比较显示S1、S2和S3计数无显著差异（P >.05）。在肿瘤组中，77%的病例S2细菌检测为阳性，S3为68%。在对照组中，S2和S3的相应数字均为65%。两组之间链球菌和放线菌的患病率、水平及对治疗的反应无显著差异（P >.05）。
使用旋转器械、次氯酸钠冲洗和氢氧化钙用药进行根管治疗在降低癌症患者患有根尖周炎的感染牙齿中的细菌数量方面非常有效，与对照组无显著差异。
