一种可在高离心力下可视化细胞内结构的离心偏光显微镜（CPM）。

A Centrifuge Polarizing Microscope (CPM) That Enables the Visualization of Intracellular Structures Under High Centrifugal Forces.

机构信息

Institute of Photonics Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan.

Marine Biological Laboratory, Woods Hole, MA, USA.

出版信息

Methods Mol Biol. 2025;2872:75-85. doi: 10.1007/978-1-0716-4224-5_5.

DOI:10.1007/978-1-0716-4224-5_5

PMID:39616569

Abstract

The mitotic spindle consists of aligned filaments of dynamic microtubules that faithfully segregate mitotic chromosomes. This view of the mitotic spindle was initially established by detecting weak birefringence of the aligned filaments, which was realized by developing polarized light microscopy (Inoué S, Chromosoma 5:487-500. https://doi.org/10.1007/bf01271498 , 1953). Inoué et al. developed a centrifuge polarizing microscope (CPM) (Inoué S et al., J Microsc 201:341-356. https://doi.org/10.1046/j.1365-2818.2001.00850.x , 2001; Inoué S et al., Centrifuge microscope capable of realizing polarized light observation (US Patent: US1907803A), 1999) to further understand the structural and physical basis of the functional positioning of centrosome-based structures (including the mitotic spindle) inside living cells. This chapter describes the procedure for observing biological specimens using CPM by focusing on Caenorhabditis elegans embryos.

摘要

有丝分裂纺锤体由排列整齐的动态微管丝组成，能准确地将有丝分裂染色体分离。这种有丝分裂纺锤体的观点最初是通过检测排列整齐的细丝的弱双折射来建立的，这是通过开发偏光显微镜（Inoué S，Chromosoma 5:487-500. https://doi.org/10.1007/bf01271498，1953）来实现的。Inoué 等人开发了一种离心式偏光显微镜（CPM）（Inoué S 等人，J Microsc 201:341-356. https://doi.org/10.1046/j.1365-2818.2001.00850.x，2001；Inoué S 等人，Centrifuge microscope capable of realizing polarized light observation (US Patent: US1907803A)，1999），以进一步了解基于中心体的结构（包括有丝分裂纺锤体）在活细胞内的功能定位的结构和物理基础。本章将重点介绍秀丽隐杆线虫胚胎，描述使用 CPM 观察生物标本的过程。