利用活细胞成像评估哺乳动物细胞中的染色体振荡。
Assessment of Chromosome Oscillations in Mammalian Cells by Live Cell Imaging.
机构信息
Department of Molecular Oncology, Institute of Development, Aging and Cancer (IDAC), Tohoku University, Sendai, Miyagi, Japan.
出版信息
Methods Mol Biol. 2025;2872:157-164. doi: 10.1007/978-1-0716-4224-5_11.
PMID:39616575
Abstract
In metaphase, chromosomes undergo a back-and-forth movement between two spindle poles called chromosome oscillation. This dynamic is necessary to maintain the robustness of chromosome segregation. This chapter describes the materials and methods required to observe chromosome oscillation in mammalian cell lines and calculate the deviation of amplitude (DAP), which is used to quantify the degree of chromosome oscillation.
摘要
在中期，染色体在两个纺锤体极之间进行往返运动，称为染色体振荡。这种动态对于维持染色体分离的稳健性是必要的。本章描述了观察哺乳动物细胞系中染色体振荡并计算幅度偏差（DAP）所需的材料和方法，该方法用于量化染色体振荡的程度。
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