Zheng Yao, Li Jiajia, Gao Jiancao, Jin Wei, Hu Jiawen, Sun Yi, Zhu Haojun, Xu Gangchun
Wuxi Fishery College, Nanjing Agricultural University, Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), Wuxi, Jiangsu 214081, China; College of Fisheries, Tianjin Agricultural University, Wuxi, Jiangsu 214081, China.
Wuxi Fishery College, Nanjing Agricultural University, Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), Wuxi, Jiangsu 214081, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2025 Mar;53:101370. doi: 10.1016/j.cbd.2024.101370. Epub 2024 Nov 28.
Studies showed that toxicants that adhered to the surface of nano-microplastics (NPs) have toxicological effects. Juvenile tilapia were divided into four groups namely the control group (A), 100 ng·L sulfamethoxazole (SMZ) group (B), 75 nm NPs group (C) and SMZ + 75 nm NPs group (D), and were exposed to an acute test for 2, 4 and 8 days. The hepatic histopathological changes, enzymatic activities, transcriptomics and proteomics analysis have been performed. The results showed that; the enzymatic activities of anti-oxidative enzymes (ROS, SOD, EROD), energy (ATP), lipid metabolism (TC, TG, FAS, LPL, ACC), pro-inflammatory factors (TNFα, IL-1β) and apoptosis (Caspase 3) have decreased significantly at 8 d. Hepatic histopathological results revealed the narrowed hepatic sinuses, displaced nucleus, and vacuoles under SMZ exposure. Transcriptome results demonstrated that endocytosis, MAPK signaling pathway, apoptosis, lysosome and herpes simplex infection were enriched in group C at 8 d. apaf1, casp3a, nfkbiaa (apoptosis, except for 8 d) were significantly increased, il1b and tgfb3, fgfr2 showed significant increase and decrease in group C/D. ctsd and ctsk associated with apoptosis have been especially significantly increased at 8 d, while MAPK signaling pathway, gadd45ga, gadd45gb/gadd45gg have been significantly decreased and increased, as well as map3k3/map3k2 significantly decreased at 8 d. Apoptosis and MAPK signaling pathway were affected and the synergistic effect was verified in tilapia liver following NPs and SMZ acute co-exposure.
研究表明，附着在纳米微塑料（NPs）表面的毒物具有毒理学效应。将幼龄罗非鱼分为四组，即对照组（A）、100 ng·L磺胺甲恶唑（SMZ）组（B）、75 nm NPs组（C）和SMZ + 75 nm NPs组（D），并进行2、4和8天的急性试验。进行了肝脏组织病理学变化、酶活性、转录组学和蛋白质组学分析。结果表明：在第8天时，抗氧化酶（ROS、SOD、EROD）、能量（ATP）、脂质代谢（TC、TG、FAS、LPL、ACC）、促炎因子（TNFα、IL-1β）和凋亡（Caspase 3）的酶活性显著降低。肝脏组织病理学结果显示，在SMZ暴露下肝窦变窄、细胞核移位和出现空泡。转录组结果表明，在第8天时，C组中内吞作用、MAPK信号通路、凋亡、溶酶体和单纯疱疹感染相关基因富集。apaf1、casp3a、nfkbiaa（凋亡，8天除外）在C组显著增加，il1b和tgfb3、fgfr2在C/D组显示出显著增加和降低。与凋亡相关的ctsd和ctsk在第8天时尤其显著增加，而MAPK信号通路、gadd45ga、gadd45gb/gadd45gg在第8天时显著降低和增加，以及map3k3/map3k2在第8天时显著降低。在NPs和SMZ急性联合暴露后，罗非鱼肝脏中的凋亡和MAPK信号通路受到影响，并验证了协同效应。
