Zheng Yao, Li Jiajia, Zhu Haojun, Hu Jiawen, Sun Yi, Xu Gangchun
Wuxi Fishery College, Nanjing Agricultural University, Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), Wuxi, Jiangsu 214081, China.
Wuxi Fishery College, Nanjing Agricultural University, Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), Wuxi, Jiangsu 214081, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2025 Mar;289:110117. doi: 10.1016/j.cbpc.2024.110117. Epub 2024 Dec 25.
Studies showed that contaminants adhered to the surface of nano-polystyrene microplastics (NPs) have a toxicological effect. Juveniles tilapia were dispersed into four groups: the control group A, 75 nm NPs exposed group B, 5 ng·L 2,2',4,4',5,5'-hexabromodiphenyl ether group C (BDE), and 5 ng·L BDE + 75 nm MPs group D, and acutely exposed for 2, 4 and 8 days. The hepatic histopathological change, enzymatic activities, transcriptomics, and proteomics, have been performed in tilapia. The results showed that the enzymatic activities of anti-oxidative (ROS, SOD, EROD), energy (ATP), lipid metabolism (TC, TG, FAS, LPL, ACC), pro-inflammatory (TNFα, IL-1β) and apoptosis (caspase 3) significantly increased at 2 d in BDE and the combined group and together in BDE group at 8 d. Histological slice showed displaced nucleus by BDE exposure and vacuoles appeared in the combined groups. KEGG results revealed that pathways associated with endocytosis, protein processing in endoplasmic reticulum and regulation of actin cytoskeleton were significantly enriched. The selected genes associated with neurocentral development (ganab, diaph3/baiap2a/ddost decreased and increased), lipid metabolism (ldlrap1a decreased, stt3b increased), energy (agap2 decreased, uggt1 increased) were affected under co-exposure, and fibronectin significantly increased via proteome. Our study indicated that endocytosis, protein processing in endoplasmic reticulum, regulation of actin cytoskeleton were affected in tilapia liver under NPs and BDE co-exposure.
研究表明，附着在纳米聚苯乙烯微塑料（NPs）表面的污染物具有毒理学效应。将罗非鱼幼鱼分为四组：对照组A、75纳米NPs暴露组B、5纳克·升2,2',4,4',5,5'-六溴二苯醚组C（BDE）和5纳克·升BDE + 75纳米微塑料组D，并进行2、4和8天的急性暴露。对罗非鱼进行了肝脏组织病理学变化、酶活性、转录组学和蛋白质组学研究。结果表明，抗氧化（ROS、SOD、EROD）、能量（ATP）、脂质代谢（TC、TG、FAS、LPL、ACC）、促炎（TNFα、IL-1β）和凋亡（caspase 3）的酶活性在BDE组和联合组中于第2天显著增加，在BDE组中于第8天共同显著增加。组织切片显示BDE暴露导致细胞核移位，联合组出现空泡。KEGG结果显示，与内吞作用、内质网中的蛋白质加工和肌动蛋白细胞骨架调节相关的途径显著富集。在共同暴露下，与神经中枢发育相关的选定基因（ganab、diaph3/baiap2a/ddost减少和增加）、脂质代谢（ldlrap1a减少，stt3b增加）、能量（agap2减少，uggt1增加）受到影响，并且通过蛋白质组学发现纤连蛋白显著增加。我们的研究表明，在NPs和BDE共同暴露下，罗非鱼肝脏中的内吞作用、内质网中的蛋白质加工、肌动蛋白细胞骨架调节受到影响。
