A novel type III effector RipBU from Ralstonia solanacearum suppresses plant immunity and promotes peanut susceptibility.

A predicted peanut R. solanacearum T3E RS_T3E_Hyp6 was identified as a definite T3E and renamed as RipBU. It is relative conserved in 31 R. solanacearum strains. Deletion of RipBU in R. solanacearum HA4-1 strain caused the attenuate pathogenicity in peanut, and complementarity of RipBU recovered the virulence of ΔRipBU mutant strain. Transient expression of RipBU decreased the level of chlorophyll, resulting in leaf chlorosis and suppressed flg22-triggered reactive oxygen species (ROS) burst and the expression of pattern-triggered immunity (PTI) marker genes in the leaves of Nicotiania benthamiana. Subcellular localization observation showed that RipBU localizes to chloroplasts in tobacco cells. RipBU significantly increased the jasmonic acid (JA) content and the expressions of JA-signaling marker genes in tobacco leaves, while significantly decreased the salicylic acid (SA) level and the expressions of SA-signaling marker genes. RipBU contained a putative lipase domain, and mutation of which abolished the ability of RipBU to induce tobacco leaf chlorosis and peanut wilt, while still localized to chloroplasts. Our study reveals the virulence function of RipBU that suppresses plant immunity by inhibiting PTI and SA signaling, and promoting JA signaling.