Skjønsberg O H, Kierulf P, Gravem K, Fagerhol M K, Godal H C
Thromb Res. 1986 Jan 1;41(1):1-8. doi: 10.1016/0049-3848(86)90274-4.
In order to estimate the solubility of contaminating fibrin in CPD-blood, thrombin induced fibrin polymerzation in CPD-plasma was examined by light scattering and fibrinopeptide A (FPA) determinations. In addition, I125 fibrin monomer enriched CPD-blood was used to investigate fibrin monomer retention in blood bags and transfusion filters (170 microns) and fibrin distribution in blood components derived from CPD-blood. Initial fibrin polymerization in CPD-blood occurred after conversion of 15 per cent of the fibrinogen to fibrin, implying that substantial amounts of fibrin may be kept solubilized in CPD-blood bags. Only minor amounts of I125 fibrin monomers were retained in blood bags (2.4 per cent) and in transfusion filters (2.9 per cent) after sham transfusions. After separating I125-fibrin monomer enriched CPD-blood into its constituent components, the major part of fibrin (75.0 per cent) could be traced in the cryoprecipitate.
为了评估CPD血液中污染性纤维蛋白的溶解度，通过光散射和纤维蛋白肽A（FPA）测定来检测凝血酶诱导CPD血浆中的纤维蛋白聚合。此外，使用富含I125纤维蛋白单体的CPD血液来研究血袋和输血过滤器（170微米）中纤维蛋白单体的保留情况以及CPD血液衍生的血液成分中纤维蛋白的分布。CPD血液中最初的纤维蛋白聚合发生在15%的纤维蛋白原转化为纤维蛋白之后，这意味着大量的纤维蛋白可能在CPD血袋中保持溶解状态。模拟输血后，只有少量的I125纤维蛋白单体保留在血袋中（2.4%）和输血过滤器中（2.9%）。将富含I125纤维蛋白单体的CPD血液分离成其组成成分后，大部分纤维蛋白（75.0%）可以在冷沉淀中检测到。
