Xiaojing Sun, Li Ma
Department of Ophthalmology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
Department of Ophthalmology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
Int J Biol Macromol. 2025 Jan;286:138235. doi: 10.1016/j.ijbiomac.2024.138235. Epub 2024 Nov 30.
Diabetic retinopathy is a common microvascular complication in diabetic patients, which can lead to blindness in severe cases. At present, although a variety of treatment methods are available, the pathological mechanism has not been fully elucidated. As ribosomal proteins, RPL3 and MRPL16 play important roles in cell metabolism and protein synthesis, but their specific roles in diabetic retinopathy are unclear. This study aims to construct new features of diabetic retinopathy by WGCNA method, and reveal the mechanism of RPL3 and MRPL16 protein in diabetic retinopathy, so as to provide new ideas for the early diagnosis and treatment of diabetic retinopathy. The study collected retinal tissue samples from patients with diabetic retinopathy and used high-throughput sequencing techniques to obtain gene expression data. Gene expression data were analyzed by WGCNA method to construct the characteristic module of diabetic retinopathy. On this basis, the genes significantly associated with diabetic retinopathy were screened out, and the mechanism of action of RPL3 and MRPL16 proteins in diabetic retinopathy was studied through bioinformatics analysis and experimental verification. Through WGCNA analysis, we successfully constructed a characteristic module of diabetic retinopathy and screened out genes significantly associated with the disease. Further studies have shown that RPL3 and MRPL16 proteins are up-regulated in diabetic retinopathy and play key roles in cell metabolism and protein synthesis. Through in vitro experiments and animal model verification, we found that the abnormal expression of RPL3 and MRPL16 proteins is closely related to the pathological process of diabetic retinopathy.
糖尿病视网膜病变是糖尿病患者常见的微血管并发症，严重时可导致失明。目前，虽然有多种治疗方法，但病理机制尚未完全阐明。作为核糖体蛋白，RPL3和MRPL16在细胞代谢和蛋白质合成中发挥重要作用，但其在糖尿病视网膜病变中的具体作用尚不清楚。本研究旨在通过WGCNA方法构建糖尿病视网膜病变的新特征，揭示RPL3和MRPL16蛋白在糖尿病视网膜病变中的作用机制，为糖尿病视网膜病变的早期诊断和治疗提供新思路。该研究收集了糖尿病视网膜病变患者的视网膜组织样本，并使用高通量测序技术获得基因表达数据。通过WGCNA方法对基因表达数据进行分析，构建糖尿病视网膜病变的特征模块。在此基础上，筛选出与糖尿病视网膜病变显著相关的基因，并通过生物信息学分析和实验验证研究RPL3和MRPL16蛋白在糖尿病视网膜病变中的作用机制。通过WGCNA分析，我们成功构建了糖尿病视网膜病变的特征模块，并筛选出与该疾病显著相关的基因。进一步研究表明，RPL3和MRPL16蛋白在糖尿病视网膜病变中上调，并在细胞代谢和蛋白质合成中起关键作用。通过体外实验和动物模型验证，我们发现RPL3和MRPL16蛋白的异常表达与糖尿病视网膜病变的病理过程密切相关。
