四磨汤治疗功能性消化不良大鼠模型肠道微生物群落组成分析
Analysis of composition of gut microbial community in a rat model of functional dyspepsia treated with Simo Tang.
作者信息
Yiying Wang, Jianjun Liu, Yongjian Xiong, Yongli Zhang, Yuqi Wen, Mengli Xue, Huishu Guo, Juanjuan Qiu
机构信息
Centralab, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
Clinical Laboratory of Integrative Medicine, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
出版信息
J Tradit Chin Med. 2024 Dec;44(6):1168-1176. doi: 10.19852/j.cnki.jtcm.20240927.003.
OBJECTIVE
To investigate composition of gut microbial community in a rat model of functional dyspepsia (FD) and to explore the interventional effects of Simo Tang (, SMT).
METHODS
A rat model of FD was established through the tail-clamping stimulation method. The rat model of FD was assessed by the state of rats, their weight, sucrose preference rate, and intestinal propulsion rate. The DNA was extracted from stool samples after treatment with SMT. Amplified polymerase chain reaction (PCR) products of the 16S rDNA were sequenced using NovaseQ6000 after construction of libraries. Composition of gut microbial community in the stool samples was determined and analyzed by cluster analysis, bioinformatic analysis, and analysis of α-diversity and β-diversity.
RESULTS
The rat model of FD was successfully established using the tail-clamping stimulation method. The statistical results of cluster analysis of operational taxonomic units (OTUs) showed that the relative abundance of OTUs in the FD group was the lowest, while it was the highest in the normal (N) group. The composition of microbiome in the four groups was similar at phyla level. Compared with the FD group, the abundance of Firmicutes was downregulated, and the abundance of Proteobacteria and Bacteroidetes was upregulated in the Simo Tang (SMT) and high-dose Simo Tang (SMT.G) groups. The ratio of Bacteroidetes/ Firmicutes was also elevated. According to the analysis of α-diversity and β-diversity, the abundance of flora in FD rats was significantly reduced. The treatment using SMT appeared beneficial to improve the diversity of flora. SMT could improve the intestinal flora in FD rats. The results showed that FD rats had intestinal flora imbalance, and species diversity increased. The results suggested that SMT could regulate the disorders of intestinal flora caused by FD.
CONCLUDIONS
SMT could restore gut homeostasis and maintain gut flora diversity by modulating the gut microbiota and its associated metabolites in rats, thereby treating gastrointestinal diseases.
目的
研究功能性消化不良（FD）大鼠模型肠道微生物群落组成，并探讨四磨汤（SMT）的干预作用。
方法
采用夹尾刺激法建立FD大鼠模型。通过大鼠状态、体重、蔗糖偏好率和肠道推进率对FD大鼠模型进行评估。用SMT处理后从粪便样本中提取DNA。构建文库后，使用NovaseQ6000对16S rDNA的聚合酶链反应（PCR）扩增产物进行测序。通过聚类分析、生物信息学分析以及α多样性和β多样性分析，确定并分析粪便样本中肠道微生物群落的组成。
结果
采用夹尾刺激法成功建立了FD大鼠模型。可操作分类单元（OTUs）聚类分析的统计结果显示，FD组OTUs的相对丰度最低，而正常（N）组最高。四组微生物群落的组成在门水平上相似。与FD组相比，四磨汤（SMT）组和高剂量四磨汤（SMT.G）组中厚壁菌门的丰度下调，变形菌门和拟杆菌门的丰度上调。拟杆菌门/厚壁菌门的比例也升高。根据α多样性和β多样性分析，FD大鼠的菌群丰度显著降低。使用SMT治疗有利于改善菌群多样性。SMT可改善FD大鼠的肠道菌群。结果表明，FD大鼠存在肠道菌群失衡，物种多样性增加。结果提示，SMT可调节FD引起的肠道菌群紊乱。
结论
SMT可通过调节大鼠肠道微生物群及其相关代谢产物恢复肠道稳态，维持肠道菌群多样性，从而治疗胃肠道疾病。
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