Singh Gurtej, Kaymakcalan Omer, Hays Thomas R, Abbas Adam, Sridevan Sruthi, Kaur Jasleen, Rathi Sourish, Marquez Jocellie, Ghazizadeh Soosan, Tavernier Felix, Boyce Brendan F, Khan Sami U, Khan Fazel A, Bui Duc T, Dagum Alexander B
From the Division of Plastic and Reconstructive Surgery, Department of Surgery, Stony Brook University Hospital and Medical Center, Stony Brook, NY.
Orlando Health at Orlando Regional Medical Center, Orlando, FL.
Plast Reconstr Surg Glob Open. 2024 Nov 27;12(11):e6317. doi: 10.1097/GOX.0000000000006317. eCollection 2024 Nov.
One reason for local recurrence is the presence of positive surgical margins after tumor resection. An animal model accurately representing the microtumor burden will improve our understanding of these surgical margins. Using a rat model, we report a new methodology for creating microscopic tumors.
Four different cell densities (1000, 10,000, 100,000, and 500,000 cells) of virus-induced Rous sarcoma XC cells (ATCC CCL-165) were topically added to 1.0 cm × 1.0 cm full-thickness wounds of male Rowett nude rats. Both Tegaderm and Tensoplast were then used to dress the wounds. After 9 days, the wound beds were excised, stained with hematoxylin and eosin, and analyzed using National Institutes of Health Image J software.
Better healing of the wound beds was observed for the smaller 2 cell densities (1000 and 10,000) as opposed to the higher 2 densities (100,000 and 500,000). The 2 higher cell density groups had gross identifiable tumors that extended deep through the dermis. On the other hand, the smaller cell density groups had microscopic tumor masses. Inflammation was present in all groups irrespective of the initial tumor cell densities, whereas hemorrhage was present only in the 2 higher cell density groups.
This methodology can create a clinically relevant scenario of positive surgical margins after tumor resection. This induction method is simple, reasonably quick to use, and requires minimal surgical expertise. This approach could also develop microscopically positive margins for a much more comprehensive array of cancers.
局部复发的一个原因是肿瘤切除后手术切缘阳性。一个能准确反映微小肿瘤负荷的动物模型将增进我们对这些手术切缘的理解。我们利用大鼠模型报告了一种创建微小肿瘤的新方法。
将病毒诱导的劳氏肉瘤XC细胞（ATCC CCL - 165）的四种不同细胞密度（1000、10000、100000和500000个细胞）局部添加到雄性罗维特裸鼠1.0 cm×1.0 cm的全层伤口上。然后分别用特洁肤和弹力绷带包扎伤口。9天后，切除伤口床，用苏木精和伊红染色，并使用美国国立卫生研究院图像J软件进行分析。
与较高的两种细胞密度（100000和500000）相比，较低的两种细胞密度（1000和10000）的伤口床愈合情况更好。较高细胞密度的两组有肉眼可识别的肿瘤，深入真皮层。另一方面，较低细胞密度的两组有微小肿瘤团块。无论初始肿瘤细胞密度如何，所有组均有炎症，而出血仅出现在较高细胞密度的两组中。
该方法可创建肿瘤切除后手术切缘阳性的临床相关情况。这种诱导方法简单，使用起来相当快捷，且所需手术专业知识最少。这种方法还可为更广泛的一系列癌症形成微小阳性切缘。
