Chyrek-Tomaszewska Aleksandra, Popiołek Alicja, Stachowicz-Karpińska Agnieszka, Budzyński Jacek, Linkowska Katarzyna, Kozakiewicz Mariusz, Szelągowski Adam, Borkowska Alina, Bieliński Maciej
Chair and Department of Clinical Neuropsychology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland.
Department of Vascular and Internal Diseases, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland.
Front Endocrinol (Lausanne). 2024 Nov 15;15:1417292. doi: 10.3389/fendo.2024.1417292. eCollection 2024.
While numerous studies have established associations between brain-derived neurotrophic factor (BDNF) and cognitive functioning, limited research has delved into the role of BDNF concerning cognitive outcomes in atherosclerosis-related conditions. This study aimed to investigate the correlations between cognitive performance, serum BDNF levels, and the BDNF Val66Met polymorphism in individuals diagnosed with chronic limb ischemia (CLI).
The study encompassed 159 CLI patients (52 females, 107 males) aged 59-73 years. Genetic analysis involved assessing the BDNF Val66Met single-nucleotide polymorphism using a TaqMan SNP Genotyping Assay and the ViiA™ 7 Real-Time PCR System. Serum BDNF levels were quantified. Cognitive functioning was evaluated through a computerized battery of tests, including the simple reaction test (SRT) for speed and accuracy assessment, verbal memory test (VMT) for short and long-term memory evaluation, and the GoNoGo test for cognitive control and inhibition.
Gender differences in cognitive performance were observed, with women excelling in VMT, while men demonstrated superior performance in SRT and the GoNoGo test. No statistically significant differences were noted between the Met/Met or Met/Val and Val/Val subgroups. However, notable differences emerged in female Met carriers, exhibiting superior VMT scores but more incorrect Go responses in the GoNoGo test. Conversely, female Val homozygotes showed significantly more incorrect NoGo responses compared to male Val homozygotes. In men carrying the Met allele, higher BDNF concentrations correlated with improved GoNoGo test results (r = 0.248, p = 0.01). Conversely, in women with the Val/Val variant, lower BDNF concentrations were associated with better VMT scores.
This study underscores the sex-specific impact of BDNF serum levels and the BDNF polymorphism on cognitive processes among CLI patients. The findings highlight the nuanced influence of BDNF in shaping cognitive functioning, emphasizing the need for further research into these sex-dependent associations.
虽然众多研究已确立脑源性神经营养因子（BDNF）与认知功能之间的关联，但关于BDNF在动脉粥样硬化相关病症的认知结果方面的作用，研究仍很有限。本研究旨在调查被诊断为慢性肢体缺血（CLI）的个体的认知表现、血清BDNF水平和BDNF Val66Met基因多态性之间的相关性。
该研究纳入了159名年龄在59 - 73岁之间的CLI患者（52名女性，107名男性）。基因分析包括使用TaqMan SNP基因分型检测法和ViiA™ 7实时PCR系统评估BDNF Val66Met单核苷酸多态性。对血清BDNF水平进行了定量。通过一组计算机化测试评估认知功能，包括用于速度和准确性评估的简单反应测试（SRT）、用于短期和长期记忆评估的言语记忆测试（VMT）以及用于认知控制和抑制的Go/NoGo测试。
观察到认知表现存在性别差异，女性在VMT方面表现出色，而男性在SRT和Go/NoGo测试中表现更优。Met/Met或Met/Val与Val/Val亚组之间未观察到统计学上的显著差异。然而，女性Met携带者出现了显著差异，她们在VMT中得分更高，但在Go/NoGo测试中Go反应错误更多。相反，女性Val纯合子与男性Val纯合子相比，NoGo反应错误明显更多。在携带Met等位基因的男性中，较高的BDNF浓度与更好的Go/NoGo测试结果相关（r = 0.248，p = 0.01）。相反，在具有Val/Val变体的女性中，较低的BDNF浓度与更好的VMT得分相关。
本研究强调了BDNF血清水平和BDNF多态性对CLI患者认知过程的性别特异性影响。研究结果突出了BDNF在塑造认知功能方面的细微影响，强调需要对这些性别依赖性关联进行进一步研究。
