Basabrain Mohammed S, Zaeneldin Ahmed, Bijle Mohammed Nadeem, Zhang Chengfei
Restorative Dental Sciences, Faculty of Dentistry, Umm Al-Qura University, Makkah, Saudi Arabia.
Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong, SAR, China.
Heliyon. 2024 Nov 8;10(22):e40262. doi: 10.1016/j.heliyon.2024.e40262. eCollection 2024 Nov 30.
Dental stem cells with neurosphere-forming abilities are a promising cell source for the treatment of neural diseases and injuries. This scoping review aimed to systematically map the existing literature on dental sphere formation assays and their characteristics associated with neural regeneration potential.
The Web of Science, EMBASE, SCOPUS, and PubMed databases were systematically searched for , animal, and clinical studies and reviews focusing on stem cells isolated from the oral cavity, subsequently cultured as spheres with neural regeneration potential. Data were extracted and evidence was synthesized according to the predetermined variables in the registered protocol.
A total of 35 articles (31 , 1 combined and , and 3 reviews) were included. The predominant method utilized for sphere formation was low-attachment culture. Spheres were characterized using assessment of neural marker expression confocal microscopy, immunohistochemistry, RT-qPCR, or western blotting. Overall, the synthesized results indicate a lack of studies investigating the utility of dental neurospheres for neural regeneration, with dental pulp stem cells being the most investigated for their neural regenerative potential.
Dental stem cell spheres demonstrate significant potential for neural regeneration. Several assays and characterizations have been performed to characterized the mechanisms underlying dental sphere formation. Furthermore, studies are imperative to deduce the neural regenerative potential of stem cells in complex biological environments.
具有形成神经球能力的牙干细胞是治疗神经疾病和损伤的一种有前景的细胞来源。本综述旨在系统梳理关于牙球形成测定及其与神经再生潜能相关特征的现有文献。
系统检索科学网、EMBASE、SCOPUS和PubMed数据库，查找专注于从口腔分离的干细胞，随后培养成具有神经再生潜能的球状体的实验研究、动物研究和临床研究及综述。根据注册方案中的预定变量提取数据并综合证据。
共纳入35篇文章（31篇实验研究、1篇综合实验研究和动物研究以及3篇综述）。形成球状体的主要方法是低贴壁培养。使用共聚焦显微镜、免疫组织化学、RT-qPCR或蛋白质免疫印迹法评估神经标志物表达来表征球状体。总体而言，综合结果表明缺乏研究调查牙神经球用于神经再生的效用，牙髓干细胞因其神经再生潜能是研究最多的。
牙干细胞球状体显示出显著的神经再生潜能。已经进行了多种测定和表征来阐明牙球形成的潜在机制。此外，迫切需要开展实验研究以推断复杂生物环境中干细胞的神经再生潜能。
