Enhanced biosorption of cadmium ions on immobilized surface-engineered yeast using cadmium-binding peptides.

A new type of cadmium (Cd) ion cell surface adsorbent was developed by integrating bacteriophage display peptide library technology with cell surface display technology. Cd chelating resin served as the target molecule in screening experiments, leading to the identification of four Cd -binding peptides. These peptides were introduced into via the pYD1 plasmid using lithium acetate heat shock transformation. Adsorption efficiency tests indicated that the engineered yeasts adsorbed more Cd than the control strain EBY100 when exposed to the same amount of Cd. Among these peptides, sequence 3-containing strain was demonstrated to have the highest Cd adsorption efficiency, being 35% higher than the control strain. Additionally, when this recombinant yeast strain was immobilized using sodium alginate, the adsorption efficiency was increased by 55.7% compared to the control strain.