Li Hua, Dong Wei, Liu Yong, Zhang Haiyan, Wang Gang
Institute of Microbial Engineering, Henan University, Kaifeng, China.
School of Life Sciencel, Henan University, Kaifeng, China.
Front Microbiol. 2019 Jun 26;10:1254. doi: 10.3389/fmicb.2019.01254. eCollection 2019.
Three nickel-binding peptides were screened from a phage peptide library and displayed separately or in combination with surface-engineered EBY100. The sorption of nickel ions on the surface of yeast cells increased with the increasing number of nickel Ni(II)-binding peptides displayed. The combined expression of the three peptides by EBY100/pYD1-N123 demonstrated the highest sorption of Ni(II) (2.603 ± 0.004 g g, dry weight) and an enhanced sorption capacity of 60.15%, compared to EBY100. An orthogonal test for yeast immobilization was designed. A maximum sorption capability of 68.62% was observed for a treatment at 25°C with 2.0% calcium chloride and 3.0% sodium alginate.
从噬菌体肽库中筛选出三种镍结合肽，并分别展示或与表面工程化的EBY100组合展示。随着展示的镍Ni(II)结合肽数量增加，酵母细胞表面对镍离子的吸附量增加。EBY100/pYD1-N123对三种肽的组合表达显示出对Ni(II)的最高吸附量（2.603±0.004 g g，干重），与EBY100相比，吸附能力提高了60.15%。设计了酵母固定化的正交试验。在25°C下用2.0%氯化钙和3.0%海藻酸钠处理时，观察到最大吸附能力为68.62%。
