Huang Rong, Meng Qingxin, Xun Lijie, Wu Xiaoman, Yue Dan, Zhao Wenzheng, Dong Xia, Gong Xueyang, Dong Kun
Yunnan Provincial Engineering and Research Center for Sustainable Utilization of Honey Bee Resources, Eastern Bee Research Institute, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, China.
Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi, China.
Front Plant Sci. 2024 Nov 15;15:1494855. doi: 10.3389/fpls.2024.1494855. eCollection 2024.
Endophytic fungi are extensive in plant tissues and involved in the defense against stress from harmful microbes. The interaction between pedicel endophytic fungi and nectar yeasts is critical for maintaining nectar homeostasis. This study used as the research subject. High-throughput sequencing revealed that the community composition of endophytic fungi in the pedicel is dominated by Ascomycota and Basidiomycota. Their abundance varies at different taxonomic levels, showing sample variability. In total, 27 endophytic fungal isolates were isolated and screened from the pedicel under laboratory conditions. They exhibited antagonistic effects against three nectar yeasts (, , and ) and displayed morphological and physiological diversity. The isolates were classified into the phylum Ascomycota and further categorized into the genera , , , and . The endophytic fungus D23, which effectively antagonizes nectar yeasts, was identified as . This fungus produces various secondary metabolites, including antibiotics such as penicillin G, grandiomycin, and cephalosporin C. The metabolic pathways involved include the biosynthesis of plant secondary metabolites, phenylpropanoids, amino acids, nucleotides, and antibiotics. The endophytic fungal community in pedicel is rich and diverse, making it a valuable material for screening antagonistic strains. This study provides a theoretical basis for the antagonistic effects of endophytic fungal metabolites from the pedicel of against nectar yeasts, highlighting their significance in maintaining nectar stability and reproductive fitness in cross-pollinated plants.
内生真菌广泛存在于植物组织中，并参与抵御来自有害微生物的胁迫。花梗内生真菌与花蜜酵母之间的相互作用对于维持花蜜稳态至关重要。本研究以[具体植物名称未给出]为研究对象。高通量测序显示，花梗内生真菌的群落组成以子囊菌门和担子菌门为主。它们在不同分类水平上的丰度有所不同，呈现出样本变异性。在实验室条件下，共从花梗中分离筛选出27株内生真菌菌株。它们对三种花蜜酵母（[具体酵母名称未给出]、[具体酵母名称未给出]和[具体酵母名称未给出]）表现出拮抗作用，并呈现出形态和生理多样性。这些分离菌株被归类为子囊菌门，进一步分为[具体属名未给出]、[具体属名未给出]、[具体属名未给出]和[具体属名未给出]属。有效拮抗花蜜酵母的内生真菌D23被鉴定为[具体菌种未给出]。该真菌产生多种次生代谢产物，包括青霉素G、宏大霉素和头孢菌素C等抗生素。涉及的代谢途径包括植物次生代谢产物、苯丙烷类、氨基酸、核苷酸和抗生素的生物合成。[具体植物名称未给出]花梗中的内生真菌群落丰富多样，使其成为筛选拮抗菌株的宝贵材料。本研究为[具体植物名称未给出]花梗内生真菌代谢产物对花蜜酵母的拮抗作用提供了理论依据，突出了它们在维持异花授粉植物花蜜稳定性和繁殖适合度方面的重要性。
