Departamento de Farmácia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, MG, Brazil.
Faculdade de Medicina, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, MG, Brazil.
J Appl Microbiol. 2020 Oct;129(4):926-934. doi: 10.1111/jam.14666. Epub 2020 May 3.
This study aimed to isolate Pseudobrickellia brasiliensis endophytic bacteria and evaluate the production of hydrolytic enzymes and antibiotics by these bacterial strains. The study also measured the antibacterial activity of P. brasiliensis.
Thirteen endophytic bacteria strains were isolated from stem and leaf fragments of P. brasiliensis. Extracellular enzyme production by the isolated endophytic bacteria was evaluated in an agar plate-based assay. The highest protease production was achieved by Bacillus subtilis P4 in alkaline medium. Antimicrobial activity of endophytic bacteria and P. brasiliensis extracts was investigated using microbroth dilution. An MIC value of 1000 μg ml against Pseudomonas aeruginosa was found for B. subtilis P3, B. subtilis P5, Pseudomonas sp. P8 and Pseudomonas sp. P12. Leaf extract of P. brasiliensis showed the highest antibacterial activity against P. aeruginosa, with an MIC value of 0·781 mg ml .
Pseudobrickellia brasiliensis is a source of bacterial endophytes, which can produce antibacterial compounds and enzymes. This work also demonstrated the antibacterial potential of P. brasiliensis.
This is the first study that revealed the antibacterial activity of P. brasiliensis and bioactive metabolite production by P. brasiliensis endophytic bacteria.
本研究旨在从巴西鳞花木茎和叶的组织碎片中分离内生细菌，并评估这些菌株产水解酶和抗生素的能力，同时还测定了巴西鳞花木的抑菌活性。
从巴西鳞花木的茎和叶组织碎片中分离出 13 株内生细菌。采用平板法测定分离内生细菌的胞外酶的产生情况。在碱性介质中，枯草芽孢杆菌 P4 产生的蛋白酶活性最高。采用微量稀释法研究内生细菌和巴西鳞花木提取物的抑菌活性。枯草芽孢杆菌 P3、枯草芽孢杆菌 P5、假单胞菌 P8 和假单胞菌 P12 对铜绿假单胞菌的 MIC 值均为 1000μg/ml。巴西鳞花木叶提取物对铜绿假单胞菌的抑菌活性最高，MIC 值为 0.781mg/ml。
巴西鳞花木是内生细菌的来源，内生细菌可产生抗菌化合物和酶。本研究还证实了巴西鳞花木的抑菌潜力。
这是首次揭示巴西鳞花木的抑菌活性和巴西鳞花木内生细菌产生的生物活性代谢产物。
