Interplays of Dietary Fat with BMI and rs324420 on HDL-C in Gender-Dependent Manner in Adolescents.

The present study was to explore relationship between serum lipid profiles and the polymorphism of rs324420 at fatty acid amide hydrolase (FAAH) gene ( rs324420) and its confounders in Chinese adolescents. Serum lipids, glucose, and insulin levels were assessed using routine methods in a cohort of 708 high school students. Dietary intake was investigated by 3-day diet records, and intakes of protein, fat, and carbohydrate were calculated. rs324420 was genotyped by polymerase chain reaction-restriction fragment length polymorphism technique and confirmed through DNA sequencing. In the whole study population, increased HDL-C levels were observed in rs324420 CC homozygotes than those in A allele carriers. Body mass index (BMI), gender, intake of fat, and rs324420 were predictive factors of HDL-C levels in the whole study population. Moreover, BMI and intake of fat were predictors of HDL-C levels in male rs324420 A allele carriers and female CC homozygotes, but only BMI was predictor of HDL-C in female A allele carriers and male CC homozygotes. These results demonstrate that there are mutual effects of dietary fat with gender, BMI, and rs324420 on HDL-C levels, which pave a novel way to explore the heterogeneous relationship of serum lipid profiles with diets or rs324420 and provide a new perspective of precision dietary interventions of dyslipidemia, especially in adolescents.