Üstün Selime, Kizil Hamit Emre, Dertli Enes
Department of Food Engineering Bayburt University Bayburt Türkiye.
Vocational School of Health Services Bayburt University Bayburt Türkiye.
Food Sci Nutr. 2024 Oct 14;12(11):9469-9478. doi: 10.1002/fsn3.4507. eCollection 2024 Nov.
The aim of this study was to evaluate the cytotoxic effects of enzymatically modified pectin products derived from grapefruit, jujube, and kumquat on the MKN-45 gastric cancer cell line in vitro. FTIR analysis revealed that the spectra of the pectins produced were comparable to those of commercial pectin and included the characteristic peaks identified in the literature. The galacturonic acid content was measured as 612 mg/g in grapefruit pectin, 544 mg/g in jujube pectin, and 704 mg/g in kumquat pectin. DSC analysis indicated that all pectin samples and their modifications exhibited one endothermic peak and one exothermic peak. Cytotoxicity assessments revealed that the enzymatically modified grapefruit pectin demonstrated significant cytotoxic effects across all tested concentrations, with 0.075 mg/mL being the most effective. For kumquat pectin, all tested concentrations showed cytotoxic properties, with 0.3, 0.15, and 0.075 mg/mL being the most effective. In the case of jujube pectin, cytotoxic effects were observed at all concentrations except for 1.2 mg/mL.
本研究的目的是评估源自葡萄柚、枣和金橘的酶法改性果胶产品对MKN - 45胃癌细胞系的体外细胞毒性作用。傅里叶变换红外光谱（FTIR）分析表明，所制备果胶的光谱与商业果胶的光谱相当，且包含文献中鉴定出的特征峰。测得葡萄柚果胶中的半乳糖醛酸含量为612 mg/g，枣果胶为544 mg/g，金橘果胶为704 mg/g。差示扫描量热法（DSC）分析表明，所有果胶样品及其改性产物均呈现一个吸热峰和一个放热峰。细胞毒性评估显示，酶法改性的葡萄柚果胶在所有测试浓度下均表现出显著的细胞毒性作用，其中0.075 mg/mL最为有效。对于金橘果胶，所有测试浓度均显示出细胞毒性特性，0.3、0.15和0.075 mg/mL最为有效。对于枣果胶，除1.2 mg/mL外，在所有浓度下均观察到细胞毒性作用。
