Banerjee Shefali, Jewell Kelsea A, Brennan Greg
Department of Microbiology, Immunology and Cancer Biology, School of Medicine, University of Virginia, Charlottesville, VA, USA.
Biology Department, Centralia College, Centralia, WA, USA.
Methods Mol Biol. 2025;2860:241-256. doi: 10.1007/978-1-0716-4160-6_16.
Experimental evolution is the process of exposing virus populations to defined selective pressures in a laboratory setting to identify adaptive changes. Coupled with deep sequencing, this experimental approach allows for nucleotide-level resolution of poxvirus adaptive strategies over time. Here, we present a general method of poxvirus experimental evolution, Illumina-based deep sequencing, and bioinformatic analyses to identify structural changes (e.g., gene duplication) as well as local adaptive changes (e.g., small indels and single nucleotide polymorphisms).
实验进化是指在实验室环境中使病毒群体暴露于特定选择压力下以识别适应性变化的过程。结合深度测序，这种实验方法能够随着时间推移在核苷酸水平解析痘病毒的适应性策略。在此，我们展示了一种痘病毒实验进化、基于Illumina的深度测序及生物信息学分析的通用方法，以识别结构变化（如基因重复）以及局部适应性变化（如小的插入缺失和单核苷酸多态性）。
