Wang Ting-Ching, Dollahon Christina R, Mishra Sneha, Patel Hailee, Abolghasemzade Samere, Singh Ishita, Thomazy Vilmos, Rosen Daniel G, Sandulache Vlad C, Chakraborty Saptarshi, Lele Tanmay P
Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX, USA.
Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA.
NPJ Precis Oncol. 2024 Dec 2;8(1):276. doi: 10.1038/s41698-024-00775-8.
Nuclear atypia is a hallmark of cancer. A recent model posits that excess surface area, visible as folds/wrinkles in the lamina of a rounded nucleus, allows the nucleus to take on diverse shapes with little mechanical resistance. Whether this model is applicable to normal and cancer nuclei in human tissues is unclear. We image nuclear lamins in patient tissues and find: (a) nuclear laminar wrinkles are present in control and cancer tissue but are obscured in hematoxylin and eosin (H&E) images, (b) nuclei rarely have a smooth lamina, and (c) wrinkled nuclei assume diverse shapes. Deep learning reveals the presence of extreme nuclear laminar wrinkling in cancer tissues, which is confirmed by Fourier analysis. These data support a model in which excess surface area in the nuclear lamina enables nuclear shape diversity in vivo. Extreme laminar wrinkling is a marker of cancer, and imaging the lamina may benefit cancer diagnosis.
核异型性是癌症的一个标志。最近的一个模型认为，多余的表面积，在圆形细胞核的核膜中表现为褶皱/皱纹，使细胞核能够以很小的机械阻力呈现出多样的形状。该模型是否适用于人体组织中的正常细胞核和癌细胞核尚不清楚。我们对患者组织中的核纤层蛋白进行成像，发现：（a）对照组织和癌组织中都存在核膜皱纹，但在苏木精和伊红（H&E）图像中不明显，（b）细胞核很少有光滑的核膜，（c）有皱纹的细胞核呈现出多样的形状。深度学习揭示了癌组织中存在极端的核膜皱纹，这一点通过傅里叶分析得到了证实。这些数据支持了一个模型，即核膜中多余的表面积能够在体内实现细胞核形状的多样性。极端的核膜皱纹是癌症的一个标志物，对核膜进行成像可能有助于癌症诊断。
