Yao H, Liu K, Liu C, Li W, Dai Q, Zhao S, Ding Z, Wang H, Ge X, Wei P, Duan J, Xi M
School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, China.
Xi'an TANK Medicinal Biology Institute, Xi'an 710032, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2024 Nov 20;44(11):2121-2130. doi: 10.12122/j.issn.1673-4254.2024.11.08.
To explore the therapeutic mechanism of maggot for psoriasis-like lesions in mice from the perspective of immune stress and complement activation regulation.
Thirty-six male C57BL/6 mice were randomly divided into control group, model group, maggot (1.25%, 2.5%, and 5%) groups, and Benvitimod (1%) group. Psoriasis-like lesions were induced by application of imiquimod cream, and the severity of skin lesions was assessed using the modified Psoriasis Area and Severity Index (MPASI) score. Auricular swelling of the mice was observed, and histopathological changes of the skin lesions were examined with HE staining. Scratching behavior of the mice was observed and the spleen index was calculated. Toluidine blue staining was used to detect mast cells in the skin lesions, and serum levels of IgG, IgM, the complements CH50, C1s, C3, C3a, C5 and C5a, and the inflammatory factors IL-23, IL-17A and TNF-α were determined with ELISA.
In mice with imiquimod-induced psoriasis-like skin lesions, treatment with the maggot at the 3 doses significantly decreased MPASI score, alleviated auricular swelling and pathologies in the skin lesions, reduced scratching behaviors, spleen index, and the number of mast cells in the lesions. Treatment with high-dose maggot significantly lowered serum levels of IgG, C1s, C3a, C5a, IL-23, IL-17A and TNF- α and the levels of C1s, C3, C3a, C5 and C5a in the lesion tissue, and increased serum levels of CH50, C3, and C5. The therapeutic effect of maggot showed a dose-effect dependence.
Maggot can alleviate psoriasislike skin lesions in mice by inhibiting immune stress and complement activation.
从免疫应激和补体激活调节的角度探讨蛆虫对小鼠银屑病样皮损的治疗机制。
将36只雄性C57BL/6小鼠随机分为对照组、模型组、蛆虫（1.25%、2.5%和5%）组和本维莫德（1%）组。通过涂抹咪喹莫特乳膏诱导银屑病样皮损，采用改良的银屑病面积和严重程度指数（MPASI）评分评估皮肤损伤的严重程度。观察小鼠耳部肿胀情况，并用苏木精-伊红（HE）染色检查皮肤损伤的组织病理学变化。观察小鼠搔抓行为并计算脾脏指数。用甲苯胺蓝染色检测皮肤损伤中的肥大细胞，并用酶联免疫吸附测定（ELISA）法测定血清中免疫球蛋白G（IgG）、免疫球蛋白M（IgM）、补体CH50、C1s、C3、C3a、C5和C5a以及炎症因子白细胞介素-23（IL-23）、白细胞介素-17A（IL-17A）和肿瘤坏死因子-α（TNF-α）的水平。
在咪喹莫特诱导的银屑病样皮肤损伤小鼠中，3种剂量的蛆虫治疗均显著降低MPASI评分，减轻耳部肿胀和皮肤损伤病理变化，减少搔抓行为、脾脏指数及损伤部位肥大细胞数量。高剂量蛆虫治疗显著降低血清中IgG、C1s、C3a、C5a、IL-23、IL-17A和TNF-α水平以及损伤组织中C1s、C3、C3a、C5和C5a水平，并提高血清中CH50、C3和C5水平。蛆虫的治疗效果呈剂量效应依赖性。
蛆虫可通过抑制免疫应激和补体激活减轻小鼠银屑病样皮肤损伤。
