Tan Panlin, Mei Jianbing, Wang Dong
Department of Orthopaedics, People's Hospital of Linshui, Linshui, 638500, China.
Department of Radiology, Chongqing University Cancer Hospital, Chongqing, China.
J Orthop Surg Res. 2024 Dec 3;19(1):816. doi: 10.1186/s13018-024-05313-7.
To explore the therapeutic effects of phellodendrine on non-compression lumbar disc herniation (NCLDH).
The Sprague Dawley rat model of NCLDH was established via autologous caudal nucleus pulposus transplantation. Behavioral observations and neurological function scoring were conducted in Sprague Dawley rats, and the serum levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were measured via enzyme-linked immunosorbent assay (ELISA). Real-time quantitative polymerase chain reaction (RT‒qPCR) was used to detect the expression of nuclear factor kappa-B (NF-κB) p65 mRNA in L5 nerve roots and surrounding tissues. Western blotting was used to assess the protein expression of NF-κB p65 and TNF-α. Immunofluorescence and immunohistochemical analyses were performed to investigate the distribution and expression of the NF-κB p65 protein in the L5 nerve and its surrounding tissues.
In this animal study, phellodendrine was found to downregulate the expression of p65 mRNA, decrease the release of inflammatory factors, and alleviate motor dysfunction caused by lumbar disc herniation(LDH). Therefore, the phellodendrine technique has potential value for the treatment of NCLDH.
In this animal experiment, phellodendrine was found to significantly reduce the expression level of p65 mRNA, decrease the release of inflammatory cytokines, and alleviate lumbar disc pain.
Not applicable.
探讨黄柏碱对非压迫性腰椎间盘突出症（NCLDH）的治疗作用。
通过自体尾核髓核移植建立NCLDH的Sprague Dawley大鼠模型。对Sprague Dawley大鼠进行行为观察和神经功能评分，并通过酶联免疫吸附测定（ELISA）测量血清肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）水平。采用实时定量聚合酶链反应（RT-qPCR）检测L5神经根及周围组织中核因子κB（NF-κB）p65 mRNA的表达。采用蛋白质免疫印迹法评估NF-κB p65和TNF-α的蛋白表达。进行免疫荧光和免疫组织化学分析，以研究NF-κB p65蛋白在L5神经及其周围组织中的分布和表达。
在本动物研究中，发现黄柏碱可下调p65 mRNA的表达，减少炎症因子的释放，并减轻腰椎间盘突出症（LDH）引起的运动功能障碍。因此，黄柏碱技术对NCLDH的治疗具有潜在价值。
在本动物实验中，发现黄柏碱可显著降低p65 mRNA的表达水平，减少炎性细胞因子的释放，并减轻腰椎间盘疼痛。
不适用。
