Sönmezer Açıkgöz Dilek, Latifoğlu Fatma, Toprak Güler, Baran Münevver
Department of Biomedical Engineering, University of Cukurova, Adana, Turkey.
Department of Biomedical Engineering, University of Erciyes, Kayseri, Turkey.
Biotechnol Appl Biochem. 2024 Dec 2. doi: 10.1002/bab.2697.
Enhancing the biocompatibility of biomaterials is a critical aspect of tissue engineering and regenerative medicine. Advances in 3D bioprinting technology, blending natural and synthetic materials for the production of bioink, offer new opportunities to develop highly biocompatible materials that can closely mimic the native tissue environment. In this study, we used pericardial fluid structure (PFS)-based material together with alginate to mimic the extracellular matrix (ECM) and produce a bioink material. Thus, blended alginate with PFS material and MC3T3-E1 pre-osteoblast cell-laden hydrogels characterized by comparing each other, especially alginate hydrogels, and evaluated in terms of biocompatibility for tissue engineering applications. According to the rheological analysis results, all hydrogel groups A, A-PFS (150 mg), and A-PFS (1:1) had viscoelastic properties. Mechanical tests showed that the A-PFS (1:1) hydrogel had the most strength properties. Additionally, the viscosity values of the hydrogel solutions were in an applicable range for use in 3D bioprinters. It was also found out that PFS increased the biocompatibility of alginate-based bioink, in terms of cell proliferation and differentiation. Overall, these findings suggest that alginate and pericardial fluid-based materials can be successfully used for bioink production. The resulting hydrogels exhibit viscoelastic properties, appropriate viscosity for 3D bioprinting, and support cell viability, proliferation, and osteogenic differentiation. This research has the potential not only to produce bioink but also to produce injectable hydrogels and drug delivery systems, which can become biocompatible materials that can be used for tissue engineering and regenerative medicine applications.
提高生物材料的生物相容性是组织工程和再生医学的一个关键方面。3D生物打印技术的进步，即将天然材料和合成材料混合用于生物墨水的生产，为开发能够紧密模拟天然组织环境的高生物相容性材料提供了新机会。在本研究中，我们使用基于心包液结构（PFS）的材料与藻酸盐一起模拟细胞外基质（ECM）并生产一种生物墨水材料。因此，将藻酸盐与PFS材料混合，并对负载MC3T3-E1前成骨细胞的水凝胶进行相互比较，特别是藻酸盐水凝胶，并对其在组织工程应用中的生物相容性进行评估。根据流变学分析结果，所有水凝胶组A、A-PFS（150mg）和A-PFS（1:1）都具有粘弹性。力学测试表明，A-PFS（1:1）水凝胶具有最强的强度性能。此外，水凝胶溶液的粘度值在适用于3D生物打印机的范围内。还发现，就细胞增殖和分化而言，PFS提高了藻酸盐基生物墨水的生物相容性。总体而言，这些发现表明藻酸盐和基于心包液的材料可成功用于生物墨水生产。所得水凝胶具有粘弹性、适合3D生物打印的粘度，并支持细胞活力、增殖和成骨分化。这项研究不仅有潜力生产生物墨水，还能生产可注射水凝胶和药物递送系统，这些都可以成为用于组织工程和再生医学应用的生物相容性材料。
