Verdier Maxime, Boissinot Sylvaine, Baltenweck Raymonde, Negrel Lise, Brault Véronique, Ziegler-Graff Véronique, Hugueney Philippe, Scheidecker Daniele, Krieger Célia, Chesnais Quentin, Drucker Martin
INRAE, Université de Strasbourg, SVQV, Colmar, France.
CNRS, Université de Strasbourg, Institut de Biologie Moléculaire des Plantes, Strasbourg, France.
Plant Cell Environ. 2025 Mar;48(3):2434-2444. doi: 10.1111/pce.15303. Epub 2024 Dec 2.
Many plant viruses modify the phenotype of their hosts, which may influence the behaviour of their vectors and facilitate transmission. Among them is the turnip yellows virus (TuYV), which can modify the orientation, feeding, and performance of its main aphid vector, Myzus persicae. However, the virus factors driving these mechanisms have not been elucidated. In this study, we compared the feeding behaviour and fecundity of aphids on TuYV-infected and transgenic Arabidopsis thaliana expressing individual TuYV proteins (CP, RT and P0) to define the role of these proteins in aphid-plant interactions. Aphids on TuYV-infected plants had shorter pathway phases and ingested phloem sap for longer times, which is expected to promote the acquisition of the phloem-limited TuYV. No change in aphid fecundity was observed on TuYV-infected plants. The transmission-conducive feeding behaviour changes could be fully reproduced by phloem-specific expression of the capsid protein (CP) in transgenic plants, whereas expression of P0 had minor and RT had no effects on aphid feeding behaviour. We then carried out a metabolomic analysis to determine plant compounds that could be involved in the modification of the aphid behaviour. A few metabolites were specific for TuYV-infected or CP-transgenic A. thaliana, and are good candidates for inducing behavioural changes.
许多植物病毒会改变其宿主的表型，这可能会影响其传播媒介的行为并促进病毒传播。其中包括芜菁黄化病毒（TuYV），它可以改变其主要蚜虫传播媒介桃蚜的取食方向、取食行为和生存能力。然而，驱动这些机制的病毒因子尚未阐明。在本研究中，我们比较了蚜虫在感染TuYV的拟南芥和表达单个TuYV蛋白（CP、RT和P0）的转基因拟南芥上的取食行为和繁殖力，以确定这些蛋白在蚜虫与植物相互作用中的作用。感染TuYV的植物上的蚜虫路径阶段较短，吸食韧皮部汁液的时间更长，这有望促进获取韧皮部受限的TuYV。在感染TuYV的植物上未观察到蚜虫繁殖力的变化。衣壳蛋白（CP）在转基因植物中的韧皮部特异性表达可完全重现有利于病毒传播的取食行为变化，而P0的表达对蚜虫取食行为影响较小，RT则无影响。然后，我们进行了代谢组学分析，以确定可能参与改变蚜虫行为的植物化合物。一些代谢物是感染TuYV的或CP转基因拟南芥特有的，是诱导行为变化的良好候选物。
