Li Fei, Wei Jiaqing, Ren Xiaoxuan, Hao Yawei, Tian Shuaishuai, Li Lizhi
Department of Orthopaedics, Second Hospital of Shanxi Medical University, No. 382, Wuyi Road, Taiyuan, 030001, PR China.
Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, 030001, PR China.
Heliyon. 2024 Sep 10;10(22):e37744. doi: 10.1016/j.heliyon.2024.e37744. eCollection 2024 Nov 30.
Osteosarcoma (OS) is a primary malignant bone tumor in children and young adults because of its intertumoral heterogeneity and absence of molecular targets. This study aimed to elucidate the role of circular RNA in the pathogenesis of OS. Using bioinformatics analysis and OS clinical samples, we found that hsa_circ_0029634 formed during the splicing process of (circZMYM2) was highly expressed in OS tissues. Nuclear cap-binding protein subunit 2 regulated circZMYM2 bio-generation by binding to flanking sequences of transcripts. In addition, knockdown circZMYM2 inhibited cell growth and metastasis in OS cell lines and inhibited tumor growth . Mechanistically, circZMYM2 competitively bound to FXR1 to regulate the translation of NACA and ARPC1B. CircZMYM2 may be a crucial regulator of OS tumorigenesis and a potential diagnostic and therapeutic biomarker for OS patients.
骨肉瘤（OS）是儿童和青年中的一种原发性恶性骨肿瘤，因其肿瘤间的异质性和缺乏分子靶点。本研究旨在阐明环状RNA在骨肉瘤发病机制中的作用。通过生物信息学分析和骨肉瘤临床样本，我们发现（circZMYM2）在剪接过程中形成的hsa_circ_0029634在骨肉瘤组织中高表达。核帽结合蛋白亚基2通过与转录本的侧翼序列结合来调节circZMYM2的生物生成。此外，敲低circZMYM2可抑制骨肉瘤细胞系中的细胞生长和转移，并抑制肿瘤生长。从机制上讲，circZMYM2竞争性结合FXR1以调节NACA和ARPC1B的翻译。CircZMYM2可能是骨肉瘤肿瘤发生的关键调节因子，也是骨肉瘤患者潜在的诊断和治疗生物标志物。
