Amoushahi Mahboobeh, Jørgensen Peter Hjorth, Kjeldgaard Anastasia Buch, Padi Eugene, Fossum Magdalena
Laboratory of Tissue Engineering, Rigshospitalet, Faculty of Clinical Medicine, University of Copenhagen, 2100 Copenhagen, Denmark.
Department of Pediatric Surgery, Center of Cancer and Organ Diseases, Copenhagen University Hospital Rigshospitalet, 2100 Copenhagen, Denmark.
Med Int (Lond). 2024 Nov 18;5(1):7. doi: 10.3892/mi.2024.206. eCollection 2025 Jan-Feb.
The aim of the present study was to summarize what is known about the role of microRNAs (miRNAs/miRs) in relation to hypospadias. Therefore, a systematic review was performed by consulting the electronic databases, MEDLINE (PubMed) and Embase. The search strategy consisted of both MeSH and free text terms, including hypospadias AND miRNA. Only articles written in the English language were included. In the selection process, three authors assessed the studies defined through the search strategy based on titles and abstracts. The authors then analyzed the complete articles and selected studies conforming to the eligibility criteria. Studies that did not align with the adopted criteria were excluded based on the limits set by the initial search strategy: Publication details, study design and participant characteristics. Articles presenting original data on miRNAs associated with hypospadias and including information on miRNA detection and analysis methods were extracted from the selected articles. The included studies presented miRNA extraction from blood samples, preputial or penile tissues, urethral epithelium and/or foreskin fibroblasts. The compiled data focused on the regulatory role of miR-494, miR-145, miR-6756-5p, miR-182, miR-200c, miR-210 and miR-1199-5p in vital cell biological processes, such as wound healing, and cell proliferation, growth and apoptosis. These results proposed that miRNAs may serve as key molecules that merit further investigation; for instance, they may be used as potential biomarkers, diagnostic markers and, eventually, as pharmaceutical agents in hypospadias.
本研究的目的是总结目前已知的微小RNA（miRNA/miR）在尿道下裂中的作用。因此，通过检索电子数据库MEDLINE（PubMed）和Embase进行了系统综述。检索策略包括医学主题词（MeSH）和自由文本词，包括尿道下裂和miRNA。仅纳入英文撰写的文章。在筛选过程中，三位作者根据标题和摘要评估通过检索策略确定的研究。然后作者分析完整的文章，并选择符合纳入标准的研究。根据初始检索策略设定的限制，即发表细节、研究设计和参与者特征，排除不符合所采用标准的研究。从所选文章中提取呈现与尿道下裂相关的miRNA原始数据并包括miRNA检测和分析方法信息的文章。纳入的研究呈现了从血液样本、包皮或阴茎组织、尿道上皮和/或包皮成纤维细胞中提取miRNA的情况。汇总的数据聚焦于miR-494、miR-145、miR-6756-5p、miR-182、miR-200c、miR-210和miR-1199-5p在伤口愈合以及细胞增殖、生长和凋亡等重要细胞生物学过程中的调节作用。这些结果表明，miRNA可能是值得进一步研究的关键分子；例如，它们可用作潜在的生物标志物、诊断标志物，并最终用作尿道下裂的药物制剂。
