Park Suji, Shim Jae-Ryong, Goh Ri-Young, Kim Dae-Hyun, Han Jin-Yeong
Department of Laboratory Medicine, Dong-A University College of Medicine, Busan, Korea.
Department of Neurology, Dong-A University College of Medicine, Busan, Korea.
Blood Res. 2024 Dec 3;59(1):40. doi: 10.1007/s44313-024-00047-1.
The diagnosis of acute ischemic stroke (AIS) can be challenging when neuroimaging findings are normal or equivocal. Neutrophil extracellular traps (NETs), particularly histone H3.1, have potential as biomarkers for AIS. This study evaluated NETs, specifically histone H3.1, as diagnostic biomarkers for AIS. This prospective study included 89 patients with AIS and 20 healthy controls. Plasma histone H3.1 levels were measured using the Nu.Q® H3.1 enzyme-linked immunosorbent assay (ELISA). Seven cytokines were analyzed using a bead-based immunoassay. Statistical analyses were used to compare histone H3.1 levels between groups and evaluate correlations with clinical parameters and cytokines. Histone H3.1 levels were significantly higher in patients with AIS (271.05 ± 33.40 ng/mL) versus controls (95.33 ± 12.86 ng/mL, p < 0.001). Multivariable logistic regression identified H3.1 as an independent risk factor for AIS (p = 0.006), with an area under the curve of 0.907. Significant correlations were found between H3.1, interleukin-6 (0.290, p = 0.013) and vascular cell adhesion molecule 1 (0.297, p = 0.011). In conclusion, the NETs H3.1 ELISA test is a reliable new diagnostic option that supports the diagnosis of AIS.
当神经影像学检查结果正常或不明确时，急性缺血性卒中（AIS）的诊断可能具有挑战性。中性粒细胞胞外陷阱（NETs），特别是组蛋白H3.1，有潜力作为AIS的生物标志物。本研究评估了NETs，特别是组蛋白H3.1，作为AIS的诊断生物标志物。这项前瞻性研究纳入了89例AIS患者和20名健康对照。使用Nu.Q® H3.1酶联免疫吸附测定（ELISA）测量血浆组蛋白H3.1水平。使用基于微珠的免疫测定法分析七种细胞因子。采用统计学分析比较各组之间的组蛋白H3.1水平，并评估其与临床参数和细胞因子的相关性。AIS患者的组蛋白H3.1水平（271.05±33.40 ng/mL）显著高于对照组（95.33±12.86 ng/mL，p<0.001）。多变量逻辑回归确定H3.1是AIS的独立危险因素（p = 0.006），曲线下面积为0.907。发现H3.1与白细胞介素-6（0.290，p = 0.013）和血管细胞黏附分子1（0.297，p = 0.011）之间存在显著相关性。总之，NETs H3.1 ELISA检测是一种可靠的新诊断方法，有助于AIS的诊断。
