Huang Zhao, Wu Kaimin, Ju Feiye, He Ran, Tang Ying, Chen Yao, He Xuliang, Zhang Jing, Nie Libo
Life Science and Chemistry College, Hunan University of Technology, Zhuzhou 412007, China.
Zhuzhou People's Hospital, Zhuzhou 412007, China.
Bioelectrochemistry. 2025 Jun;163:108857. doi: 10.1016/j.bioelechem.2024.108857. Epub 2024 Nov 28.
Early cancer diagnosis is paramount for enhancing treatment efficacy, extending patient survival, and improving the quality of life. We developed a highly sensitive electrochemical biosensor for the detection of target DNA (tDNA) associated with gastric cancer. This advancement integrates dual signal amplification strategies: bio-barcode amplification (BCA) and surface-initiated enzyme polymerization (SIEP), with copper nanoclusters (CuNCs) serving as signal labels. Silica nanoparticles (SiO) were covalently linked with polythymine (poly T) and complementary DNA to create bio-barcode probes. These probes, through hybridization, were immobilized on the reduced graphene oxide and Au nanoparticle (rGO-AuNPs) modified interface and marking the first amplification of the electrical signal. Subsequently, the extended poly T prompted by SIEP bound additional CuNCs through the combination of T-Cu, leading to a second round of signal amplification. The biosensor demonstrated a minimum detection limit of 0.13 fmol/L over a linear response range from 1 fmol/L to 1 nmol/L. It also showcased excellent specificity, repeatability, and stability, making it a promising tool for the sensitive detection of gastric cancer biomarkers.
早期癌症诊断对于提高治疗效果、延长患者生存期和改善生活质量至关重要。我们开发了一种用于检测与胃癌相关的靶DNA（tDNA）的高灵敏度电化学生物传感器。这一进展整合了双重信号放大策略：生物条形码扩增（BCA）和表面引发酶聚合（SIEP），并以铜纳米簇（CuNCs）作为信号标签。二氧化硅纳米颗粒（SiO）与聚胸腺嘧啶（poly T）和互补DNA共价连接，以创建生物条形码探针。这些探针通过杂交固定在还原氧化石墨烯和金纳米颗粒（rGO-AuNPs）修饰的界面上，并标记电信号的首次放大。随后，由SIEP引发的延伸poly T通过T-Cu的结合与额外的CuNCs结合，导致第二轮信号放大。该生物传感器在1 fmol/L至1 nmol/L的线性响应范围内显示出0.13 fmol/L的最低检测限。它还表现出优异的特异性、重复性和稳定性，使其成为一种用于敏感检测胃癌生物标志物的有前途的工具。
