Wang Xue, Xiong Shibo, Liu Yue, Chen Jia-Hao, Chen Jiahe, Shi Pengfei, Li Xuemei, Zhou Hong
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering. Qingdao University of Science and Technology, Qingdao, 266042, China.
Collaborative Innovation Center of Tumor Marker Detection Technology, Equipment and Diagnosis-Therapy Integration in Universities of Shandong, Shandong Province Key Laboratory of Detection Technology for Tumor Makers, School of Chemistry and Chemical Engineering, College of Medicine, Linyi University, Linyi, 276005, China.
Biosens Bioelectron. 2025 Mar 1;271:117011. doi: 10.1016/j.bios.2024.117011. Epub 2024 Nov 29.
Recently, organic photoelectrochemical transistor (OPECT) has become a very interesting biological measurement method in photoelectrochemical (PEC) bioanalysis and future bio-related applications. OPECT is expected to be a powerful tool for disease detection and early warning. Circulating tumor cells (CTCs) are generally deemed to be the dominant factor of tumor metastasis, and 90 % of cancer patients die from this metastatic disease. Therefore, there is an imminent need to develop a highly sensitive CTCs detection sensing system to improve the survival rate of cancer patients. Here, we use a DNA tetrahedrons (DNA NTH) with an aptamer at the top to immobilize on the surface of the photoelectric material to capture cells (MCF-7). Specifically, the BiVO-ZnInS hybrid was synthesized by a simple hydrothermal method, which can effectively modulated devices with high current gain. Au NPs were directly integrated on the electrode surface to construct an OPECT photoelectric sensing platform. Subsequently, the aptamer which is thiol-functionalized (SH-Apt) was immobilized on the electrode surface. Because of the overexpression of MUC1 protein on the cell membrane, it can specifically capture MCF-7 cells. The introduction of MCF-7 cells resulted in a significant decrease in the current signal. There is a relationship between the change of photocurrent and the logarithm of MCF-7 cell concentration, which is a good linear relationship ranging from 50 to 5 × 10 cell mL. The obtained detection limit is 43 cell mL. The biosensor has high selectivity and sensitivity, and achieves sensitive detection of MCF-7.
最近，有机光电化学晶体管（OPECT）已成为光电化学（PEC）生物分析及未来生物相关应用中一种非常有趣的生物测量方法。OPECT有望成为疾病检测和早期预警的有力工具。循环肿瘤细胞（CTC）通常被认为是肿瘤转移的主要因素，90%的癌症患者死于这种转移性疾病。因此，迫切需要开发一种高灵敏度的CTC检测传感系统，以提高癌症患者的生存率。在此，我们使用顶部带有适配体的DNA四面体（DNA NTH）固定在光电材料表面以捕获细胞（MCF-7）。具体而言，通过简单的水热法合成了BiVO-ZnInS复合材料，其能够有效调制具有高电流增益的器件。将金纳米颗粒直接集成在电极表面以构建OPECT光电传感平台。随后，将硫醇功能化的适配体（SH-Apt）固定在电极表面。由于细胞膜上MUC1蛋白的过表达，它能够特异性捕获MCF-7细胞。MCF-7细胞的引入导致电流信号显著降低。光电流变化与MCF-7细胞浓度的对数之间存在关系，在50至5×10细胞/mL范围内呈良好的线性关系。获得的检测限为43细胞/mL。该生物传感器具有高选择性和灵敏度，实现了对MCF-7的灵敏检测。
