Key Laboratory of Analysis and Detection for Food Safety (MOE and Fujian Province), Collaborative Innovation Center of Detection Technology for Haixi Food Safety and Products (Fujian Province), State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry, Fuzhou University , Fujian Province, China , 350002.
Anal Chem. 2016 Dec 20;88(24):12539-12546. doi: 10.1021/acs.analchem.6b04461. Epub 2016 Dec 7.
Photoelectrochemical (PEC) measurement has been developed rapidly for bioanalysis in recent years. However, the actual application for most existed PEC bioanalytical systems is still a challenge because the perfect solutions for sensing surface design, high-throughput detection, and portability are lacked. To successfully overcome these limitations and realize accurate, continuous screening and assessing on prognostic indicator of early stage cancer on the spot, an innovative and portable semiautomated support power-free photoelectrochemical (SP-PEC) immunosensing platform consisted with a miniature semiautomatic injection system and digital multimeter (DMM) readout is designed (prostate specific antigen, PSA, was used as the proof-of-concept analyte). Decahedral BiVO that decorated with Au nanocrystal on {010} facets (Au-BiVO) by photodeposition is used as the photoanode materials to produce photocurrent signal under irradiation of micro laser light (5.0 w, λ ≥ 380 nm). The monoclonal anti-PSA capture antibody (mAb)-functionalized FeO magnetic nanobeads (mAb-MN) and glucose oxidase (GOx)/monoclonal detection antibody (mAb)-conjugated gold nanoparticle (GOx-AuNP-mAb) are employed as immunosensing probe and signal probe, respectively. The HO as an excellent holes scavenger that in suit generated from GOx oxidization glucose substrate significantly amplifies the photocurrent. The variation of instantaneous current value that registered as the signal of the immunoassay increases linearly with the logarithm of target PSA concentration increasing in a wide range from 10 pg mL to 100 ng mL with a low detection limit (LOD) of 4.0 pg mL. The SP-PEC immunosensing platform not only simplifies the assay process, but also improves detecting efficiency. The semiautomatic and portable SP-PEC analysis device allows analysis on spot and high-throughput continuous detection. Additional, we also gain deep insight into the relations between the specific shape as well as Au nanocrystal decoration and PEC activity and speculate the possible enhancement mechanisms of Au-BiVO. Therefore, the present work not only develops a flexible SP-PEC biosensor platform for rapid and continuous detection, but also provides a possible route for designing high performance photoelectric materials.
光电化学（PEC）测量近年来在生物分析中得到了迅速发展。然而，对于大多数现有的 PEC 生物分析系统，实际应用仍然是一个挑战，因为缺乏用于传感表面设计、高通量检测和便携性的完美解决方案。为了成功克服这些限制，并实现对早期癌症预后标志物的现场准确、连续的筛选和评估，设计了一种创新的、便携的半自动无支撑光电化学（SP-PEC）免疫传感平台，该平台由微型半自动注射系统和数字多用表（DMM）读数组成（前列腺特异性抗原 PSA 被用作概念验证分析物）。通过光沉积在{010}面上修饰有 Au 纳米晶的十面体 BiVO（Au-BiVO）用作光电阳极材料，在微激光光（5.0 w，λ≥380 nm）照射下产生光电流信号。单克隆抗 PSA 捕获抗体（mAb）功能化的 FeO 磁性纳米珠（mAb-MN）和葡萄糖氧化酶（GOx）/单克隆检测抗体（mAb）-共轭金纳米颗粒（GOx-AuNP-mAb）分别用作免疫传感探针和信号探针。HO 作为一种优异的空穴清除剂，可从 GOx 氧化葡萄糖底物中原位生成，显著放大光电流。作为免疫分析信号的瞬时电流值的变化与目标 PSA 浓度的对数呈线性增加，在 10 pg mL 至 100 ng mL 的较宽范围内，检测限（LOD）为 4.0 pg mL。SP-PEC 免疫传感平台不仅简化了检测过程，而且提高了检测效率。半自动和便携式 SP-PEC 分析装置允许现场分析和高通量连续检测。此外，我们还深入了解了特定形状以及 Au 纳米晶修饰与 PEC 活性之间的关系，并推测了 Au-BiVO 可能的增强机制。因此，本工作不仅开发了一种用于快速连续检测的灵活 SP-PEC 生物传感器平台，而且为设计高性能光电材料提供了一种可能的途径。
