马粪便钙卫蛋白及钙卫蛋白：蛋白质比值的参考区间以及对患绞痛和结肠炎马匹的初步临床评估

Reference intervals for equine fecal calprotectin and calprotectin:protein ratio and preliminary clinical evaluation in horses with colic and colitis.

作者信息

Bishop Rebecca C, Shanthappa Nidhi, Connolly Sara L, Wilkins Pamela A, McCoy Annette M

出版信息

Am J Vet Res. 2024 Nov 27;86(1). doi: 10.2460/ajvr.24.09.0271. Print 2025 Jan 1.

DOI:10.2460/ajvr.24.09.0271

PMID:39626360

Abstract

OBJECTIVE

To establish the reference interval (RI) of fecal calprotectin (fCP) and fCP:protein ratio in the feces of healthy horses and demonstrate preliminary clinical utility for the quantification of intestinal inflammation.

METHODS

Feces were collected from healthy horses (n = 103) and horses with colic (n = 15) or colitis (n = 13). Feces were suspended in buffer to create fecal supernatant. Fecal calprotectin concentration was determined by ELISA, fecal total protein concentration was determined by bicinchoninic acid assay, and the fCP:protein ratio was calculated. Reference intervals for fCP and fCP:protein ratio were calculated from healthy horses. Descriptive and inferential statistics were performed to evaluate associations between fCP concentration and fCP:protein ratio with group and other clinical features. The study period was from September 20, 2023, through April 20, 2024.

RESULTS

The RI for fCP was 0.056 to 0.278 μg/mL; 4 of 13 colitis cases had fCP above the RI. The RI for fCP:protein was 6.6 X 10-6 to 4.9 X 10-5; 5 of 13 colitis cases and 2 of 15 colic cases had fCP:protein above the RI. Horses with colitis had significantly greater fCP (median [quartile 1, quartile 3], 0.234 [0.194, 0.279] μg/mL) than healthy horses (0.196 [0.138, 0.220] μg/mL) or colic cases (0.189 [0.164, 0.215] μg/mL). There was a greater difference in fCP:protein ratio between colitis cases (4.31 X 10-5 [3.19 X 10-5, 6.23 X 10-5]) and both healthy horses (2.19 X 10-5 [1.58 X 10-5, 2.88 X 10-5]) and colic cases (1.52 X 10-5 [1.22 X 10-5, 3.56 X 10-5]).

CONCLUSIONS

Both fCP and fCP:protein ratio were increased in horses in colitis; additional work is needed to establish clinical utility.

CLINICAL RELEVANCE

There are few reliable diagnostic modalities for inflammation of the equine large colon. With additional development of a clinically applicable test and validation in larger population, fecal calprotectin may facilitate noninvasive quantification of intestinal inflammation in horses.

摘要

目的

建立健康马匹粪便中粪钙卫蛋白（fCP）及fCP:蛋白比值的参考区间（RI），并证明其在肠道炎症定量方面的初步临床应用价值。

方法

收集健康马匹（n = 103）、患绞痛的马匹（n = 15）或患结肠炎的马匹（n = 13）的粪便。将粪便悬浮于缓冲液中制成粪便上清液。采用酶联免疫吸附测定法（ELISA）测定粪便钙卫蛋白浓度，采用二辛可宁酸法测定粪便总蛋白浓度，并计算fCP:蛋白比值。根据健康马匹的数据计算fCP和fCP:蛋白比值的参考区间。进行描述性和推断性统计，以评估fCP浓度和fCP:蛋白比值与组别及其他临床特征之间的关联。研究期间为2023年9月20日至2024年4月20日。

结果

fCP的RI为0.056至0.278μg/mL；13例结肠炎病例中有4例fCP高于RI。fCP:蛋白的RI为6.6×10⁻⁶至4.9×10⁻⁵；13例结肠炎病例中有5例、15例绞痛病例中有2例fCP:蛋白高于RI。患结肠炎的马匹的fCP（中位数[四分位数1，四分位数3]，0.234[0.194，0.279]μg/mL）显著高于健康马匹（0.196[0.138，0.220]μg/mL）或绞痛病例（0.189[0.164，0.215]μg/mL）。结肠炎病例与健康马匹（2.19×10⁻⁵[1.58×10⁻⁵，2.88×10⁻⁵]）和绞痛病例（1.52×10⁻⁵[1.22×10⁻⁵，3.56×10⁻⁵]）之间的fCP:蛋白比值差异更大。