通过circDnajc1/miR-27a-5p/C1qc信号轴探讨桃红四物汤治疗缺血性脑卒中损伤的机制。
Exploring the mechanism of Taohong Siwu Decoction in treating ischemic stroke injury via the circDnajc1/miR-27a-5p/C1qc signaling axis.
作者信息
Li Yumeng, Ye Qingping, Li Jingjing, Zhang Lijuan, Yu Chao, Xue Sujun, Li Shuangping, Duan Xianchun, Peng Daiyin
机构信息
Department of Pharmacy, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, 230031, China; School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China.
Department of Pharmacy, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, 230031, China; School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China; Xin 'an Medical Research Institute, Hefei, 230038, China; Key Laboratory of Xin'An Medicine, Ministry of Education, Hefei, 230012, China; Anhui Province Key Laboratory of Chinese Medicinal Formula, Hefei, 230012, China.
出版信息
Phytomedicine. 2025 Jan;136:156305. doi: 10.1016/j.phymed.2024.156305. Epub 2024 Nov 28.
BACKGROUND
Ischemic stroke (IS) is the most prevalent type of cerebrovascular disease. Taohong Siwu Decoction (THSWD) has been demonstrated to have neuroprotective benefits during Cerebral Ischemia-Reperfusion Injury (CIRI). Whether THSWD mitigates CIRI by modulating the circDnajc1/miR-27a-5p/C1qc signaling axis is not known.
OBJECTIVE
Examine how THSWD provides neuroprotective benefits in reducing the damage wrought by IS.
METHODS
We employed middle cerebral artery occlusion/reperfusion (MCAO/R) rat model and oxygen glucose deprivation/re-oxygenation (OGD/R) in vitro model. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR), Western blot (WB), and immunofluorescence analyses (IF) were utilized to detect microglial activation markers TMEM119, CD11b, and Iba1, inflammatory mediators CCL2, CCL6, IL1, IL6, IL10, and TNF-α, as well as circDnajc1, miR-27a-5p, C1qc, C3, and C5aR.
CONCLUSION
Our data suggest that THSWD can mitigate inflammatory response, inhibit microglial activation and neuron apoptosis, and exert neuroprotective effects by regulating the circDnajc1/miR-27a-5p/C1qc signaling axis.
背景
缺血性中风（IS）是最常见的脑血管疾病类型。桃红四物汤（THSWD）已被证明在脑缺血再灌注损伤（CIRI）期间具有神经保护作用。THSWD是否通过调节circDnajc1/miR-27a-5p/C1qc信号轴减轻CIRI尚不清楚。
目的
研究THSWD如何在减轻IS造成的损伤方面提供神经保护作用。
方法
我们采用大脑中动脉闭塞/再灌注（MCAO/R）大鼠模型和体外氧糖剥夺/复氧（OGD/R）模型。利用逆转录定量聚合酶链反应（RT-qPCR）、蛋白质免疫印迹法（WB）和免疫荧光分析（IF）检测小胶质细胞激活标志物TMEM119、CD11b和Iba1、炎症介质CCL2、CCL6、IL1、IL6、IL10和TNF-α，以及circDnajc1、miR-27a-5p、C1qc、C3和C5aR。
结论
我们的数据表明，THSWD可以减轻炎症反应，抑制小胶质细胞激活和神经元凋亡，并通过调节circDnajc1/miR-27a-5p/C1qc信号轴发挥神经保护作用。
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