Sun Xuejia, Wang Mengyuan, Zhang Renguo, Su Chenglin, Han Tingxi, Niu Na, Chen Ligang
College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China.
College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China.
Talanta. 2025 Apr 1;285:127305. doi: 10.1016/j.talanta.2024.127305. Epub 2024 Nov 30.
Gallic acid (GA) is a secondary metabolite derived from plant phenolics. It is essential to maintain normal physiological activities in plants facing adversity. This, in turn, helps maintain crop integrity. Consequently, the surveillance of GA levels in plants is of significant importance. This study developed a near-infrared (NIR) red fluorescent Ag/Au nanocluster sensor, utilizing glutathione and bovine serum albumin as dual ligands. This modification shifts the emission wavelength to the red spectrum, mitigating the interference from the plant's inherent fluorescence. The fluorescence resonance energy transfer (FRET) mechanism is exploited. When the presence of Ag induces the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to oxTMB, which in turn quenches the fluorescence at 650 nm. GA's abundant phenolic hydroxyl group will reduce oxTMB to TMB, so the material's fluorescence will be turned on to detect GA. The established platform can quantify a wide range of GA thanks to the above reasons. In particular, this platform has a recovery of 96.09%-104.7 % for detecting GA in tomato samples with an error of no more than 3 % and a detection limit as low as 38.29 nM. On the other hand, a combination of probes and the fluorescence platform was used to visualize GA levels in tomato leaves under drought and salt stress. This can assist in elucidating the physiological adaptations of plants to environmental changes. The groundbreaking GSH/BSA-Au/Ag nanosensor shows great potential for trace detection of GA in plants and will contribute to a deeper understanding of plant physiology.
没食子酸（GA）是一种源自植物酚类物质的次生代谢产物。对于面临逆境的植物而言，维持正常生理活动至关重要，这反过来有助于保持作物的完整性。因此，监测植物体内GA水平具有重要意义。本研究利用谷胱甘肽和牛血清白蛋白作为双配体，开发了一种近红外（NIR）红色荧光Ag/Au纳米簇传感器。这种修饰将发射波长转移到红色光谱，减轻了植物固有荧光的干扰。利用了荧光共振能量转移（FRET）机制。当Ag的存在诱导3,3',5,5'-四甲基联苯胺（TMB）氧化为oxTMB时，oxTMB会猝灭650 nm处的荧光。GA丰富的酚羟基会将oxTMB还原为TMB，因此该材料的荧光会被开启以检测GA。由于上述原因，所建立的平台能够对多种GA进行定量。特别是，该平台在检测番茄样品中的GA时回收率为96.09%-104.7%，误差不超过3%，检测限低至38.29 nM。另一方面，将探针与荧光平台相结合，用于可视化干旱和盐胁迫下番茄叶片中的GA水平。这有助于阐明植物对环境变化的生理适应性。开创性的GSH/BSA-Au/Ag纳米传感器在植物中GA的痕量检测方面显示出巨大潜力，并将有助于更深入地了解植物生理学。
