大鼠组织匀浆中组织蛋白酶B、H和L活性的测定方法。

Assay procedures for cathepsin B, H and L activities in rat tissue homogenates.

作者信息

Higashi T, Hashimoto M, Watanabe M, Yamauchi Y, Fujiwara M, Nakatsukasa H, Kobayashi M, Watanabe A, Nagashima H

出版信息

Acta Med Okayama. 1986 Feb;40(1):27-32. doi: 10.18926/AMO/31944.

DOI:10.18926/AMO/31944

Abstract

Cathepsin B, H and L activities in small amounts of rat tissue homogenates corresponding to 10 micrograms protein were determined with 7-amino-4-methyl-coumarin conjugates as substrates. A new procedure for serum cathepsin H activity was also developed. High cathepsin B and H activities were found in kidney, spleen and liver. Liver cathepsin B, H and L activities in D-galactosamine-injured rats were decreased concomitantly with an increase in serum cathepsin H activity.

摘要

以7-氨基-4-甲基香豆素缀合物为底物，测定了相当于10微克蛋白质的少量大鼠组织匀浆中的组织蛋白酶B、H和L活性。还开发了一种测定血清组织蛋白酶H活性的新方法。在肾脏、脾脏和肝脏中发现了较高的组织蛋白酶B和H活性。D-半乳糖胺损伤大鼠的肝脏组织蛋白酶B、H和L活性随着血清组织蛋白酶H活性的增加而降低。