Chen Feifei, Xu Jianhe, Zheng Gaowei
State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology,Shanghai 200237, China.
Eng Microbiol. 2022 May 13;2(2):100023. doi: 10.1016/j.engmic.2022.100023. eCollection 2022 Jun.
A multifunctional biocatalyst EneIRED capable of catalyzing amine-activated conjugate alkene reduction and subsequent reductive amination was discovered. The enzyme realized the coupling of α, β-unsaturated carbonyls with amines to efficiently synthesize a broad set of chiral amine diastereomers based on its unusual active site structure and catalytic mechanism.
发现了一种多功能生物催化剂EneIRED，它能够催化胺活化的共轭烯烃还原以及随后的还原胺化反应。基于其独特的活性位点结构和催化机制，该酶实现了α,β-不饱和羰基与胺的偶联，从而高效合成了多种手性胺非对映异构体。
