Pang Ai-Ping, Wang Haiyan, Luo Yongsheng, Zhang Funing, Wu Fu-Gen, Zhou Zhihua, Lu Zuhong, Lin Fengming
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.
Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Eng Microbiol. 2023 Jul 5;3(4):100105. doi: 10.1016/j.engmic.2023.100105. eCollection 2023 Dec.
β-glucosidases play an important role in the synthesis of cellulase in fungi, but their molecular functions and mechanisms remain unknown. We found that the 10 putative β-glucosidases investigated in facilitate cellulase production, with being the most crucial Transcriptional analysis revealed that the most affected biological processes in △ strain were cellulase synthesis, ribosome biogenesis, and RNA polymerases. Moreover, CEL3J was unconventionally transported through the endoplasmic reticulum, bypassing the Golgi apparatus, whereas overexpression altered cellulase secretion from conventional to unconventional, likely owing to the activated unconventional protein secretion pathway (UPS), as indicated by the upregulation of genes related to UPS. The mTORC1-GRASP55 signaling axis may modulate the unconventional secretion of CEL3J and cellulase. The transcriptional levels of genes associated with DNA replication, the cell cycle, and meiosis were noticeably affected by overexpressing . These data give new clues for exploring the roles of β-glucosidases and the molecular mechanisms of their unconventional secretion in fungi.
β-葡萄糖苷酶在真菌纤维素酶的合成中发挥着重要作用，但其分子功能和机制仍不清楚。我们发现，在所研究的10种假定的β-葡萄糖苷酶中，促进了纤维素酶的产生，其中最为关键。转录分析表明，△菌株中受影响最大的生物学过程是纤维素酶合成、核糖体生物发生和RNA聚合酶。此外，CEL3J通过内质网进行非常规运输，绕过了高尔基体，而其过表达改变了纤维素酶从常规分泌到非常规分泌的方式，这可能是由于非常规蛋白分泌途径（UPS）被激活，这一点可通过与UPS相关基因的上调来表明。mTORC1-GRASP55信号轴可能调节CEL3J和纤维素酶的非常规分泌。过表达显著影响了与DNA复制、细胞周期和减数分裂相关基因的转录水平。这些数据为探索β-葡萄糖苷酶的作用及其在真菌中非常规分泌的分子机制提供了新线索。
