Sariboga Ruken, Sarioglu Omer Faruk
Istanbul Medeniyet University, Department of Molecular Biology and Genetics, Istanbul 34730, Turkey.
Istanbul Medeniyet University, Science and Advanced Technologies Research Center (BILTAM), Istanbul 34730, Turkey.
Eng Microbiol. 2024 Aug 3;4(3):100164. doi: 10.1016/j.engmic.2024.100164. eCollection 2024 Sep.
A novel cellulolytic bacterial strain, ROBY, was isolated from a bovine rumen sample using the enrichment culture method. This isolate was found to be , with >99 % similarity according to 16S rRNA gene sequence analysis. The potential use of this strain in combination with doxorubicin (Dox)-integrated cellulose nanoparticles (Dox-CNPs) was evaluated as a proof-of-concept study for the further development of this approach as a novel controlled-release drug delivery strategy. The isolate can utilize CNPs as the sole carbon source for growth and degrade both Dox-CNPs and empty CNPs with high efficiency. Extracellular cellulases isolated from bacteria may also be used to trigger Dox release. The results also demonstrated that the release of Dox into the environment due to nanoparticle degradation in the samples incubated with Dox-CNPs significantly affected bacterial cell viability (∼75 % decrease), proving the release of Dox due to bacterial cellulase activity and suggesting the great potential of this approach for further development.
采用富集培养法从牛瘤胃样本中分离出一种新型纤维素分解细菌菌株ROBY。根据16S rRNA基因序列分析，该分离株的相似度>99%。作为将该方法进一步开发为新型控释药物递送策略的概念验证研究，评估了该菌株与阿霉素（Dox）整合纤维素纳米颗粒（Dox-CNPs）联合使用的潜在用途。该分离株可利用CNPs作为唯一碳源进行生长，并高效降解Dox-CNPs和空白CNPs。从细菌中分离出的细胞外纤维素酶也可用于触发阿霉素释放。结果还表明，在与Dox-CNPs孵育的样本中，由于纳米颗粒降解导致阿霉素释放到环境中，这显著影响了细菌细胞活力（约降低75%），证明了由于细菌纤维素酶活性导致阿霉素释放，并表明该方法具有进一步开发的巨大潜力。
