Guo Bing-Yi, Qi Yu-Ting, Wu Hui-Qian, Zha Ru-Yan, Wang Li-Jun, Zhang Xin-Wei, Huang Wei-Hua
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
Chem Commun (Camb). 2024 Dec 17;61(1):97-100. doi: 10.1039/d4cc05423g.
Reactive oxygen and nitrogen species (ROS/RNS) in macrophages have a potent killing effect on pathogens that infect the host. Here, we achieved , quantitative detection of the homeostasis of four primary ROS/RNS (ONOO, HO, NO, and NO) and their precursors (O˙, NO) in phagolysosomes of single RAW 264.7 macrophages after phagocytosis of with platinum-black nanoelectrodes. Enhanced bactericidal activity of the macrophages was observed by an increase in the total amount of ROS/RNS as well as the level and proportion of ONOO, a potent bactericidal species of RNS. Moreover, both the bactericidal process and the steady-state replenishment process were dominated by the production of RNS (NO-based), revealing differences in the enzyme kinetics of the bactericidal process.
巨噬细胞中的活性氧和氮物种（ROS/RNS）对感染宿主的病原体具有强大的杀伤作用。在此，我们通过铂黑纳米电极在吞噬[具体物质未给出]后，实现了对单个RAW 264.7巨噬细胞吞噬溶酶体中四种主要ROS/RNS（过氧亚硝酸盐、羟基自由基、一氧化氮和二氧化氮）及其前体（超氧阴离子、一氧化氮自由基）稳态的定量检测。通过ROS/RNS总量以及过氧亚硝酸盐（一种强大的RNS杀菌物种）的水平和比例的增加，观察到巨噬细胞杀菌活性增强。此外，杀菌过程和稳态补充过程均由RNS（基于一氧化氮）的产生主导，揭示了杀菌过程中酶动力学的差异。
