Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.
State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
Angew Chem Int Ed Engl. 2017 Oct 9;56(42):12997-13000. doi: 10.1002/anie.201707187. Epub 2017 Sep 8.
Nanoelectrodes allow precise and quantitative measurements of important biological processes at the single living-cell level in real time. Cylindrical nanowire electrodes (NWEs) required for intracellular measurements create a great challenge for achieving excellent electrochemical and mechanical performances. Herein, we present a facile and robust solution to this problem based on a unique SiC-core-shell design to produce cylindrical NWEs with superior mechanical toughness provided by the SiC nano-core and an excellent electrochemical performance provided by the ultrathin carbon shell that can be used as such or platinized. The use of such NWEs for biological applications is illustrated by the first quantitative measurements of ROS/RNS in individual phagolysosomes of living macrophages. As the shell material can be varied to meet any specific detection purpose, this work opens up new opportunities to monitor quantitatively biological functions occurring inside cells and their organelles.
纳米电极可实现对单细胞水平实时重要生物过程的精确和定量测量。用于细胞内测量的圆柱形纳米线电极（NWE）在实现优异的电化学和机械性能方面带来了巨大挑战。在此，我们提出了一种基于独特的 SiC 核壳设计的简便且强大的解决方案，以生产具有 SiC 纳米核提供的卓越机械韧性和超薄碳壳提供的优异电化学性能的圆柱形 NWE，该碳壳可以原样使用或镀铂。通过对活巨噬细胞中单个吞噬溶酶体中的 ROS/RNS 进行首次定量测量，说明了此类 NWE 在生物应用中的用途。由于可以改变壳材料以满足任何特定的检测目的，因此这项工作为定量监测细胞及其细胞器内发生的生物功能开辟了新的机会。
