Ayyandurai Nagarajan, Venkatesan Sethuraman, Raman Sasikumar
Department of Physical Chemistry, University of Madras, Guindy Campus, Chennai 600025 Tamil Nadu, India.
Research and Development, New Energy Technology Centre, Lithium-Ion Division, Amara Raja Advanced Cell Technology, Nanakramguda, 500032 Hyderabad, India.
ACS Appl Bio Mater. 2024 Dec 16;7(12):8444-8455. doi: 10.1021/acsabm.4c01285. Epub 2024 Dec 4.
Creatinine is indeed a crucial biomarker for kidney diseases. In this work, a novel electrochemical biosensor based on a copper-hemin metal organic framework [Cu-hemin metal-organic framework (MOF)] nanoflake decorated with palladium (Pd) (Pd/Cu-hemin MOF) was fabricated and incorporated with creatinine deiminase (CD) on a glassy carbon electrode (GCE) for creatinine detection. The formation of a Pd/Cu-hemin MOF composite was confirmed by X-ray photoelectron spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The formation of the composite as nanoflakes is evident from the scanning electron microscopy image. The transmission electron microscopy image clarifies the decoration of palladium nanoparticles on Cu-hemin MOF surfaces. Thus, the proposed biosensor (Pd/Cu-hemin MOF/CD/GCE) electrochemical performances were studied with cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy. As a result, the Pd/Cu-hemin MOF/CD/GCE-based electrochemical detection of creatinine exhibits a broad linear range from 0 to 130 μM ( = 0.99), a low limit of detection 0.08 μM, and an excellent sensitivity of 3.2 μA μM cm. The biosensor also determines creatinine in samples of human urine with a good recovery from 99.4 to 100.8%. Thus, in this study, an electrochemical biosensing platform based on Pd/Cu-hemin MOF/CD/GCE has been designed practically for creatinine.
肌酐确实是肾脏疾病的一种关键生物标志物。在这项工作中，制备了一种基于钯（Pd）修饰的铜-血红素金属有机框架[Cu-血红素金属有机框架（MOF）]纳米片的新型电化学生物传感器，并将其与肌酐脱亚氨酶（CD）整合到玻碳电极（GCE）上用于肌酐检测。通过X射线光电子能谱、X射线衍射和傅里叶变换红外光谱证实了Pd/Cu-血红素MOF复合材料的形成。从扫描电子显微镜图像图像图像图像可以明显看出形成了纳米片状的复合材料。透射电子显微镜图像清晰地显示了钯纳米颗粒在Cu-血红素MOF表面的修饰情况。因此，采用循环伏安法、差分脉冲伏安法和电化学阻抗谱研究了所提出的生物传感器（Pd/Cu-血红素MOF/CD/GCE）的电化学性能。结果表明，基于Pd/Cu-血红素MOF/CD/GCE的肌酐电化学检测具有0至130μM的宽线性范围（ = 0.99）、0.08μM的低检测限和3.2μA μM cm的优异灵敏度。该生物传感器还能测定人尿样本中的肌酐，回收率良好，在99.4%至100.8%之间。因此，在本研究中，实际上设计了一种基于Pd/Cu-血红素MOF/CD/GCE的用于肌酐检测的电化学生物传感平台。
