Cui Hong, Cui Shuaishuai, Zhang Siyuan, Tian Qiuju, Liu Yunfeng, Zhang Ping, Wang Mingxiu, Zhang Jialing, Li Xiangjun
School of Public Health, Shanxi Medical University, 56 Xinjian South Road, Taiyuan, 030001, China.
School of Chemical Sciences, University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing, 100049, China.
Analyst. 2021 Sep 27;146(19):5951-5961. doi: 10.1039/d1an01323h.
The stability, repeatability and sensitivity of an electrochemical biosensor material are closely connected with the dispersibility of metal organic frameworks (MOFs) in aqueous media. Herein, a nanocomposite based on Cu-MOF/hemin, which is not only highly water-soluble but also simple and efficient in synthesis, was used for the construction of a non-enzymatic sensor to detect hydrogen peroxide (HO). The Cu-MOF/hemin was characterized scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS)-mapping, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS) and thermal gravimetric analysis (TGA), which indicate that hemin and the Cu-MOF were successfully combined. As a HO electrochemical biomimetic enzyme, the Cu-MOF/hemin exhibited excellent electrocatalytic performance, which was confirmed by the electrochemical experiments and chromogenic reactions, and the possible mechanism of the reactions has been deduced. The electrochemical sensor based on the biomimetic enzyme exhibited an extended linear detection range from 0.01-5.0 mM ( = 0.998), low detection limit of 4.14 μM, and high selectivity and stability under the optimized conditions. More importantly, the practical application ability of the sensor was verified by the test of HO in human serum samples and it could be used for the real-time detection of HO released from living cells with satisfactory results. Therefore, this novel nanocomposite has certain potential in preparing electrochemical sensing platforms for nonenzymatic biosensing and provides a new method for clinical diagnosis and real-time monitoring.
电化学生物传感器材料的稳定性、重复性和灵敏度与金属有机框架（MOFs）在水性介质中的分散性密切相关。在此，一种基于Cu-MOF/血红素的纳米复合材料被用于构建非酶传感器以检测过氧化氢（HO），该复合材料不仅具有高度水溶性，而且合成简单高效。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、能量色散X射线光谱（EDS）映射、X射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）、X射线光电子能谱（XPS）和热重分析（TGA）对Cu-MOF/血红素进行了表征，结果表明血红素与Cu-MOF成功结合。作为一种HO电化学仿生酶，Cu-MOF/血红素表现出优异的电催化性能，这通过电化学实验和显色反应得到证实，并推导了可能的反应机理。基于该仿生酶的电化学传感器在优化条件下表现出0.01 - 5.0 mM的扩展线性检测范围（ = 0.998）、4.14 μM的低检测限以及高选择性和稳定性。更重要的是，通过对人血清样本中HO的检测验证了该传感器的实际应用能力，并且它可用于实时检测活细胞释放的HO，结果令人满意。因此，这种新型纳米复合材料在制备用于非酶生物传感的电化学传感平台方面具有一定潜力，并为临床诊断和实时监测提供了一种新方法。
