Cao Sumei, Yang Yuxin, Ma Guangtian, Wang Jing, Qiu Kai, Song Zhigang, Wu Shugeng, Qi Guanghai, Zhang Haijun
Laboratory of Quality & Safety Risk Assessment for Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China.
Laboratory of Quality & Safety Risk Assessment for Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China; Department of Animal Science, Shandong Agricultural University, Taian, China.
Poult Sci. 2025 Jan;104(1):104586. doi: 10.1016/j.psj.2024.104586. Epub 2024 Nov 22.
This experiment was conducted to evaluate the chemical composition, apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acid (AA) in 10 different sources of soybean meal (SBM) fed to the pullets and established prediction equations based on the chemical composition of SBM. 792 Hy Line Brown hens aged 12-15 weeks were divided into 11 diet groups, the diets included one nitrogen-free diet (NFD) used to measure the basal endogenous losses of AA and 10 SBM test diets containing 35.2 % SBM as the sole source of AA. The 0.5 % titanium dioxide (TiO) was used as an indigestible marker. The results shown that there was considerable variation in crude ash (Ash), ether extract (EE), and acid detergent fiber (ADF) content in the 10 SBM samples with a coefficient of variation greater than 10 %. The AID and SID of both indispensable and dispensable AA except for the Cys and Ala among the 10 SBM differed (P < 0.05). The AID of AA was correlated with the dry matter (DM), gross energy (GE), crude protein (CP), EE, Ash, and reactive Lys (rLys) (P < 0.05), and SID of AA was correlated with the GE, CP, EE, CF, neutral detergent fiber (NDF) and rLys (P < 0.05). With R values exceeding 0.39, significant linear regression equations for the AID of Met, Phe, Lys, Asp, and Gly, as well as SID of Met, Phe, Lys, Ser and Tyr were obtained. The best fit equation for the AID of Asp was the DM, GE, Ash and rLys (R = 0.820, P = 0.042), and the SID of Lys was the GE, CP, CF, Ash, ADF and rLys (R = 0.989, P = 0.005), respectively. In conclusion, this experiment suggested that the rLys can be used to predict the AID and SID of AA in SBM with reasonable accuracy together with the basal chemical composition, and prediction equations for AID and SID could be used to evaluate the digestibility of SBM in the pullets.
本试验旨在评估10种不同来源豆粕中氨基酸的化学组成、表观回肠消化率(AID)和标准回肠消化率(SID),并基于豆粕的化学组成建立预测方程。选取792只12 - 15周龄的海兰褐蛋鸡,分为11个日粮组,日粮包括一种用于测定氨基酸基础内源损失的无氮日粮(NFD)和10种以35.2%豆粕作为唯一氨基酸来源的豆粕试验日粮。以0.5%的二氧化钛(TiO₂)作为不消化标记物。结果表明,10种豆粕样品中的粗灰分(Ash)、粗脂肪(EE)和酸性洗涤纤维(ADF)含量存在显著差异,变异系数大于10%。10种豆粕中除半胱氨酸(Cys)和丙氨酸(Ala)外,必需氨基酸和非必需氨基酸的AID和SID均存在差异(P < 0.05)。氨基酸的AID与干物质(DM)、总能(GE)、粗蛋白(CP)、EE、Ash和反应性赖氨酸(rLys)相关(P < 0.05),氨基酸的SID与GE、CP、EE、CF、中性洗涤纤维(NDF)和rLys相关(P < 0.05)。蛋氨酸(Met)、苯丙氨酸(Phe)、赖氨酸(Lys)、天冬氨酸(Asp)和甘氨酸(Gly)的AID以及蛋氨酸、苯丙氨酸、赖氨酸、丝氨酸(Ser)和酪氨酸(Tyr)的SID均获得了R值超过0.39的显著线性回归方程。天冬氨酸AID的最佳拟合方程为DM、GE、Ash和rLys(R = 0.820,P = 0.042),赖氨酸SID的最佳拟合方程为GE、CP、CF、Ash、ADF和rLys(R = 0.989,P = 0.005)。综上所述,本试验表明rLys可与基础化学组成一起用于合理准确地预测豆粕中氨基酸的AID和SID,AID和SID的预测方程可用于评估蛋鸡对豆粕的消化率。