Dawit Hewan, Mehmood Shah, Hussain Zahid, Islam Syed Rashedul, Wang Zhili, Cao Yi, Liu Xingzhu, Wang Zixun, Pei Renjun
School of Nano-Tech and Nano-Bionics, University of Science and Technology of China (USTC), Hefei 230026, PR China; CAS Key Laboratory for Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics (SINANO), Chinese Academy of Sciences, Suzhou 215123, PR China.
CAS Key Laboratory for Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics (SINANO), Chinese Academy of Sciences, Suzhou 215123, PR China.
Colloids Surf B Biointerfaces. 2025 Mar;247:114420. doi: 10.1016/j.colsurfb.2024.114420. Epub 2024 Nov 30.
Arteriovenous grafts are routinely designed to provide a deliberate connection between an artery and vein in patients during hemodialysis. The commonly used grafts present significant drawbacks such as thrombosis, bacterial infection, and biofouling which prevents their functionality. To endow hydrogels with improved anti-thrombosis, stable antifouling, and strong mechanical strength, a surface-modified nanoparticle-reinforced nanohybrid hydrogel is developed. In brief, zwitterionic sulfobetaine methacrylate (SBMA) is coated on bentonite clay (BC) nanoparticles via a simple method. BC-SBMA nanoparticles were then loaded onto sodium alginate /polyvinyl alcohol hydrogel composite. Calcium chloride (Ca) crosslinking is employed to form stable network and optimize polyvinyl alcohol/sodium alginate (PS) hydrogel composite. BC-SBMA particles were dispersed into PS hydrogel and crosslinked to form nanohybrid hydrogel (PS@BC-SBMA). The nanohybrid hydrogel was characterized for its morphological, mechanical, physicochemical, antibacterial, biocompatibility, antifouling, ex-vivo anti-thrombogenic, and in-vivo anti-inflammatory properties. The results revealed that the presence ofBC-SBMA particles boosted the mechanical strength and facilitated biocompatibility. The presence of zwitterionic polymers provided excellent antifouling properties toward blood platelets, unnecessary proteins, and bacterial strains. Hence, the cooperative effects of the nanohybrid hydrogel such as biocompatibility, antifouling, and mechanical properties lead to a desirable candidate for blood-contacting implants.
动静脉移植物通常被设计用于在血液透析患者的动脉和静脉之间建立有意的连接。常用的移植物存在诸如血栓形成、细菌感染和生物污垢等重大缺点，这会妨碍其功能。为了赋予水凝胶更好的抗血栓形成、稳定的抗污垢和强大的机械强度，开发了一种表面改性的纳米颗粒增强纳米复合水凝胶。简而言之，通过一种简单的方法将两性离子甲基丙烯酸磺酸甜菜碱（SBMA）涂覆在膨润土（BC）纳米颗粒上。然后将BC-SBMA纳米颗粒负载到海藻酸钠/聚乙烯醇水凝胶复合材料上。采用氯化钙（Ca）交联形成稳定的网络并优化聚乙烯醇/海藻酸钠（PS）水凝胶复合材料。将BC-SBMA颗粒分散到PS水凝胶中并交联形成纳米复合水凝胶（PS@BC-SBMA）。对该纳米复合水凝胶的形态、机械、物理化学、抗菌、生物相容性、抗污垢、体外抗血栓形成和体内抗炎特性进行了表征。结果表明，BC-SBMA颗粒的存在提高了机械强度并促进了生物相容性。两性离子聚合物的存在对血小板、不必要的蛋白质和细菌菌株具有优异的抗污垢性能。因此，纳米复合水凝胶的生物相容性、抗污垢和机械性能等协同效应使其成为血液接触植入物的理想候选材料。
