Nanshan District Key Lab for Biopolymers and Safety Evaluation, Shenzhen Key Laboratory of Polymer Science and Technology, Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, PR China.
John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, 02138, United States.
Carbohydr Polym. 2018 Dec 15;202:246-257. doi: 10.1016/j.carbpol.2018.08.124. Epub 2018 Sep 4.
Hydrogel with good mechanical and biological properties has great potential and promise for biomedical applications. Here we fabricated a series of novel cytocompatible chitosan (CS) based double-network (DN) and triple-network (TN) hydrogels by physically-chemically crosslinking methods. Natural polysaccharide CS with abundant resources was chosen as the first network due to its good antimicrobial activity, biocompatibility and easy cross-linking reaction. Zwitterionic sulfopropylbetaine (PDMAPS) was chosen as the second network due its good biocompatibility, antimicrobial and antifouling properties. And nonionic poly(2-hydroxyethyl acrylate) (PHEA) was chosen as the final network due to its good biocompatibility, excellent nonfouling and mechanical properties. Cross-section SEM images showed that both CS/PHEA (DN, the molar ratio of glutaraldehyde to structural unit of CS is 0.2/3.0) and CS/PDMAPS/PHEA (TN, the molar ratio of glutaraldehyde to structural unit of CS is 0.2/3.0) hydrogels exhibited a smooth and uniformly dispersed porous microstructures with pore size distribution in the range of 20∼100 μm. The largest compressive stress and tensile stress of DN hydrogels reached 84.7 MPa and 292 kPa, respectively, and largest compressive stress and tensile stress of TN hydrogels could reach 81.9 MPa and 384 kPa, respectively. Moreover, the value of failure strain for TN gels reached 1020%. Besides excellent mechanical properties, DN and TN gels exhibited good antimicrobial, cytocompatible and antifouling properties due to introduction of antimicrobial chitosan, cell anti-adhesive PDMAPS and PHEA. The combination of the excellent mechanical and biological properties of multiple network hydrogels can provide a potential pathway to develop biomedical hydrogels as promising bioapplications in wound dressing and other biomedical applications.
具有良好机械和生物性能的水凝胶在生物医学应用中具有巨大的潜力和前景。在这里，我们通过物理化学交联方法制备了一系列新型的细胞相容性壳聚糖（CS）基双网络（DN）和三网络（TN）水凝胶。由于其良好的抗菌活性、生物相容性和易于交联反应，我们选择了天然多糖 CS 作为第一网络。由于其良好的生物相容性、抗菌和抗污性能，我们选择两性离子磺丙基甜菜碱（PDMAPS）作为第二网络。由于其良好的生物相容性、优异的抗污性和机械性能，我们选择非离子聚（2-羟乙基丙烯酸酯）（PHEA）作为最终网络。横截面 SEM 图像显示，CS/PHEA（DN，戊二醛与 CS 结构单元的摩尔比为 0.2/3.0）和 CS/PDMAPS/PHEA（TN，戊二醛与 CS 结构单元的摩尔比为 0.2/3.0）水凝胶均表现出光滑且均匀分散的多孔微观结构，孔径分布在 20∼100μm 范围内。DN 水凝胶的最大压缩应力和拉伸应力分别达到 84.7 MPa 和 292 kPa，TN 水凝胶的最大压缩应力和拉伸应力分别达到 81.9 MPa 和 384 kPa。此外，TN 凝胶的失效应变为 1020%。除了具有优异的机械性能外，DN 和 TN 凝胶还由于引入了抗菌壳聚糖、细胞抗黏附 PDMAPS 和 PHEA，表现出良好的抗菌、细胞相容性和抗污性能。多网络水凝胶的优异机械性能和生物性能的结合为开发作为有前途的生物应用的生物医学水凝胶提供了潜在途径，可用于伤口敷料和其他生物医学应用。
