Xiong Zhuang, Chen Xiaodie, Zou Ziping, Peng Lianxin, Zou Liang, Liu Bingliang, Li Qiang
Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering & Technology Research Center of Coarse Cereal Industrialization, School of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan, PR China.
Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering & Technology Research Center of Coarse Cereal Industrialization, School of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan, PR China.
Bioresour Technol. 2025 Feb;418:131930. doi: 10.1016/j.biortech.2024.131930. Epub 2024 Dec 2.
In this study, the bacterium XZ-A was genetically modified using atmospheric and room temperature plasma mutagenesis (ARTP) to increase the degradation efficiency of polyethylene microplastics (PE-MPs) by up to 53.65 %. After 50 d of biodegradation, the mutagenized bacterium XZ-60S caused significant changes in the morphology, structure, thermal stability, and molecular weight of PE-MPs. The number average molecular weights and weight average molecular weights of the PE-MPs were significantly reduced by approximately 15.21 % and 4.80 %, respectively. Comparative genomic and transcriptomic analyses showed that XZ-60S had a total of 106 single nucleotide polymorphic sites, and the expression of genes encoding laccases was significantly increased; this may explain the improved degradation of PE-MPs by XZ-60S. In this study, the degradation of PE-MPs by bacteria was improved through ARTP mutagenesis, which provides a reference for selecting and breeding bacteria that are highly efficient at degrading PE-MPs.
在本研究中，利用常压室温等离子体诱变技术（ARTP）对细菌XZ-A进行基因改造，使聚乙烯微塑料（PE-MPs）的降解效率提高了53.65%。经过50天的生物降解，诱变后的细菌XZ-60S使PE-MPs的形态、结构、热稳定性和分子量发生了显著变化。PE-MPs的数均分子量和重均分子量分别显著降低了约15.21%和4.80%。比较基因组学和转录组学分析表明，XZ-60S共有106个单核苷酸多态性位点，编码漆酶的基因表达显著增加；这可能解释了XZ-60S对PE-MPs降解能力的提高。在本研究中，通过ARTP诱变提高了细菌对PE-MPs的降解能力，为筛选和培育高效降解PE-MPs的细菌提供了参考。
