Han Xiaoyue, Niu Xukai, Xu Mengyan, Feng Rui, Han Qianxi, Liu Bo, Cheng Yanfen, Yun Shaojun, Cheng Feier, Feng Cuiping, Cao Jinling
College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China.
Shanxi Aquatic Technology Promotion Service Center, Taiyuan, Shanxi 030006, China.
Int J Biol Macromol. 2025 Jan;285:138299. doi: 10.1016/j.ijbiomac.2024.138299. Epub 2024 Dec 2.
The delivery vehicles based on protein-polysaccharide-polyphenol are promising methods to encapsulate bioactive components with the aim of improving their solubility and bioavailability. In this study, we used Flammulina velutipes protein (FVP) and Flammulina velutipes soluble polysaccharides (FVSP) as raw materials and prepared FVP-FVSP and FVP-FVSP-TP composite particles loaded with tea polyphenols (TP), the high internal phase Pickering emulsions stabilized by FVP-FVSP and FVP-FVSP-TP for the delivery of β-carotene (BC) were created. FVP-FVSP-TP has more promise as Pickering emulsion stabilizer than FVP-FVSP because of the smaller particle size, proper contact angle, and lower surface tension. The optimal preparation conditions of the emulsion were 4 % particle concentration and 80 % oil phase volume fraction. The emulsions stabilized by FVP-FVSP and FVP-FVSP-TP were o/w emulsions. Compared to the emulsion stabilized by FVP-FVSP, the FVP-FVSP-TP stabilized emulsion had higher G', G″ values and viscosity and showed better thermal, centrifugal, storage and oil oxidation stability. Moreover, FVP-FVSP-TP stabilized emulsions could further enhance the retention rate and bioaccessibility of TP and β-carotene. This study provides a theoretical basis for the application of FVP and FVSP in Pickering emulsions, and a reference for the fabrication of delivery vehicles to improve the stability and bioaccessibility of bioactive substances.
基于蛋白质-多糖-多酚的递送载体是封装生物活性成分以提高其溶解度和生物利用度的有前景的方法。在本研究中，我们以金针菇蛋白（FVP）和金针菇可溶性多糖（FVSP）为原料，制备了负载茶多酚（TP）的FVP-FVSP和FVP-FVSP-TP复合颗粒，并制备了由FVP-FVSP和FVP-FVSP-TP稳定的用于递送β-胡萝卜素（BC）的高内相Pickering乳液。由于粒径更小、接触角合适和表面张力更低，FVP-FVSP-TP作为Pickering乳液稳定剂比FVP-FVSP更具前景。乳液的最佳制备条件为颗粒浓度4%和油相体积分数80%。由FVP-FVSP和FVP-FVSP-TP稳定的乳液均为水包油型乳液。与由FVP-FVSP稳定的乳液相比，由FVP-FVSP-TP稳定的乳液具有更高的G'、G''值和粘度，并且表现出更好的热稳定性、离心稳定性、储存稳定性和抗油脂氧化稳定性。此外，由FVP-FVSP-TP稳定的乳液可以进一步提高TP和β-胡萝卜素的保留率和生物可及性。本研究为FVP和FVSP在Pickering乳液中的应用提供了理论依据，并为制备递送载体以提高生物活性物质的稳定性和生物可及性提供了参考。
